Detailed information NTG0697531

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: NDR85_RS05990
• Genome accession: NZ_CP098738
• Coordinates: 1217223..1220927 (+)
• Length: 1234 a.a.
• NCBI ID: WP_044156480.1
• Uniprot ID: -
• Organism: Bacillus halotolerans strain XE48
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1212223..1225927

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NDR85_RS05975 (NDR85_05975)	-	1212672..1212986 (-)	315	WP_044156484.1	four-helix bundle copper-binding protein	-
NDR85_RS05980 (NDR85_05980)	-	1213114..1213551 (-)	438	WP_044156482.1	ferritin family protein	-
NDR85_RS05985 (NDR85_05985)	addB	1213736..1217236 (+)	3501	WP_044156481.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
NDR85_RS05990 (NDR85_05990)	addA	1217223..1220927 (+)	3705	WP_044156480.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
NDR85_RS05995 (NDR85_05995)	sbcD	1220999..1222177 (+)	1179	WP_044156478.1	exonuclease subunit SbcD	-
NDR85_RS06000 (NDR85_06000)	sbcC	1222174..1225566 (+)	3393	WP_263870940.1	exonuclease subunit SbcC	-
NDR85_RS06005 (NDR85_06005)	-	1225580..1225882 (+)	303	WP_044156476.1	hypothetical protein	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 141448.91 Da
• Isoelectric Point: 5.1265

>NTDB_id=697531 NDR85_RS05990 WP_044156480.1 1217223..1220927(+) (addA) [Bacillus halotolerans strain XE48]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPVDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGDKA
FFELVDRYTTDRHDLDLQYLVKQVYEYSRSHPDPEIWLKSFVHLYDVTEKSAIEELPFYPYVKEDIAMVLDGAKEKLQRA
LELTKAPGGPAPRAENFLDDLAQIDDLIQHQDDFNELYQRVPAVSFKRAKAVKGDEFDPVLLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHVKSLADMEPVIETLVQLVISYGKRFEAAKQEKSLIDFSDLEHYCLSILTAVNEKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAVYPENSETETELLLIDKSEEADAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFDAVEVSVVLSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLSAGDRNDELYQKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKDHQKQLAKWQASASQADWLLPEFDRYQAKTYLDFIGPALVRHRDLESLAEGGAAAVSDISGHPARFAIQMIHSYD
LLDDELEERLEEKSERLEAIRRGEPVPGPFAFEEKARERLAWKYSHHEVTQIRTKQSVSEIKRRREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTIEEAERTVQHLYEKELLTEEQQSAINIEEIVQFFHTEIGE
KLIGATWKNREVPFSLALPAKEIYPDAKEADEPLLVQGIIDCLYETEDGLYLLDYKTDRIEGKFQHGFEGAAPILKKRYG
TQIQLYTRAVEQIAQTKVKGCALYFFDGGHILTL

Nucleotide

• Length: 3705 bp

>NTDB_id=697531 NDR85_RS05990 WP_044156480.1 1217223..1220927(+) (addA) [Bacillus halotolerans strain XE48]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAGTGGAATGCCATCGTCTCAACCGGCCAGGATATCCT
TGTGGCAGCGGCGGCAGGCTCCGGTAAAACCGCTGTTCTTGTCGAAAGAATGATCCGGAAAATCACGGCGGAAGAAAATC
CGGTTGATGTAGACCGGCTTCTCGTTGTCACCTTTACAAACGCCTCAGCAGCAGAGATGAAACACCGGATCGCGGATGCA
CTGGAAAAAGAGCTGGTGAAACGCCCAGGATCGCTGCATATCCGACGCCAGCTGTCGCTATTAAACCGGGCCAGCATTTC
AACGCTTCATTCGTTTTGCCTGCAGGTGCTGAAAAAATATTACTATCTGATTGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTTCTCGGGGACGAGGTGCTTGATGAGCTGTTTGAGGATGAATATGCAAAGGGTGATAAGGCG
TTCTTTGAGCTTGTCGACCGCTACACGACTGACCGGCATGACTTAGATCTGCAATATCTCGTGAAACAGGTGTACGAGTA
TTCCCGTTCCCACCCTGATCCGGAGATATGGCTGAAAAGTTTTGTTCATTTGTATGATGTAACAGAAAAGAGCGCGATCG
AGGAACTGCCGTTTTATCCGTATGTCAAAGAAGATATTGCGATGGTGCTGGACGGAGCAAAGGAAAAGCTCCAGCGTGCC
CTTGAGCTGACAAAAGCGCCGGGCGGACCGGCGCCGCGCGCTGAAAATTTTCTCGATGATCTCGCGCAGATTGATGACCT
GATTCAGCATCAGGATGACTTCAATGAACTTTATCAGCGCGTGCCCGCTGTCTCCTTTAAGCGTGCAAAAGCGGTGAAAG
GGGACGAGTTTGATCCCGTCTTGCTTGATGAAGCGACAGACTTACGAAATGGCGCCAAAAAACTGCTTGAAAAACTCAAA
ACCGATTACTTCACCAGAAGCCCGGAGCAGCATGTAAAAAGTTTGGCTGACATGGAGCCTGTCATTGAAACACTCGTACA
GCTTGTTATCAGCTACGGGAAACGGTTTGAAGCCGCAAAGCAGGAGAAATCGCTCATCGATTTTTCTGATTTGGAGCATT
ATTGCCTGTCCATCTTGACTGCTGTGAACGAAAAAGGCGAACGTGAGCCGAGTGAGGCGGCAAGATTTTATCAAGAGCAG
TTTCATGAGGTGCTTGTCGATGAATACCAGGATACCAACCTGGTGCAGGAATCCATTCTTCAGCTCGTCACAAGCGGGCC
GGAAGAGACAGGCAATCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TTCTCTCCAAGTACAAACGGTTTACAGAGAGCGGAGAAGGTACGGGCCGGAAAATCGATTTGAACAAAAACTTTCGAAGC
AGGGCTGATATTTTAGACAGCACAAACTTTTTGTTCAAACAGCTGATGGGCGGCAAAATCGGCGAGGTTGATTATGATGA
ACAGGCTGAGCTGAAACTCGGAGCGGTGTATCCGGAAAACAGCGAAACGGAAACAGAGCTGCTGTTGATTGACAAGTCAG
AGGAGGCGGACGCAGGTGAGGAAGCGGAAGAGCTGGAAACGGTGCAGTTCGAAGCAAAAGCGATCGCCAAGGAAATACGC
AAGCTGATCTCATCGCCATTTAAGGTATATGACGGGAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTGCGATCCATGCCGTGGGCGCCGCAAATCATGGAGGAACTGAGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CATCAGGCTACTTTGATGCGGTGGAAGTGTCTGTGGTGCTTTCTGTCCTGAAGGTGATTGATAACCCGTATCAGGATATT
CCGCTTGCTTCCGTTCTGCGTTCACCGATTGTCGGTGCTGACGAGAACGAGCTGTCTTTGATCCGGCTTGAAAACAAAAA
GGCGCCGTACTATGAAGCAATGAAAGACTATCTGTCAGCCGGCGACCGAAACGATGAGCTCTATCAAAAGCTGCAAACGT
TTTACGGACATTTGCAAAAGTGGCGCGCTTTTTCGAAAAACCATTCGGTGTCCGAGCTGATCTGGGAAGTGTACCGCGAC
ACAAAATATATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCTAACTTGCGTGTCCTTTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTTCGCTTCTTGAGGTTTATCGAACGCATGCAGGAACGCGGCGATG
ATCTGGGAACGGCGAGAGCGTTAAGCGAACAGGAGGATGTTGTCCGTTTAATGACGATTCACAGCAGCAAAGGGCTCGAA
TTCCCAGTCGTTTTCACAGCGGGACTCGGCCGGAATTTCAACATGATGGATCTCAACAAATCCTACCTGCTGGACAAGGA
GCTTGGGTTTGGCACGAAATTTATCCATCCTGAATGGCGCATCAGCTATCCGACGCTTCCGCTCATCGCGATGAAGAAAA
AAATGCGCAGGGAACTGCTGTCTGAGGAATTGCGTGTCCTCTATGTTGCTTTAACGAGGGCAAAGGAAAAGCTGTTTCTT
GTAGGCTCATGCAAGGATCATCAGAAACAGCTGGCAAAATGGCAGGCATCCGCGTCCCAAGCTGACTGGCTTCTGCCGGA
ATTTGACCGCTATCAGGCGAAAACGTATTTGGATTTCATCGGACCTGCTCTTGTCAGACACAGAGACTTGGAGTCTTTGG
CTGAGGGCGGCGCGGCAGCGGTTTCTGATATATCAGGCCATCCGGCCCGCTTTGCTATTCAAATGATTCATTCATATGAT
CTGCTTGATGATGAGCTGGAAGAGAGACTGGAAGAAAAAAGCGAACGTCTTGAAGCGATCCGCCGTGGTGAACCGGTTCC
CGGCCCGTTTGCATTTGAGGAAAAAGCTCGCGAGCGGCTGGCCTGGAAGTATTCGCATCACGAGGTGACACAAATTCGCA
CGAAGCAGTCCGTTTCTGAGATTAAGAGAAGAAGAGAGTACGAAGACGAATACAGCGGGCGGGCGCCCGTTAAACCGGCT
GATGGAAGCATTCTGTACAGACGCCCGGCTTTCATGATGAAAAAAGGCCTGACAGCAGCAGAAAAAGGAACAGCTATGCA
TACGGTCATGCAGCATATTCCGCTGACACATGTGCCGACGATAGAAGAAGCGGAGCGGACAGTGCAGCATCTTTATGAAA
AAGAGCTGCTCACCGAAGAACAGCAATCCGCTATCAATATTGAGGAAATCGTTCAATTTTTCCATACGGAAATTGGGGAA
AAACTGATCGGCGCTACATGGAAGAACCGCGAAGTTCCATTCAGTTTGGCGCTTCCGGCAAAAGAGATTTATCCTGATGC
GAAAGAGGCGGATGAGCCGCTATTGGTGCAGGGGATCATCGATTGTCTCTACGAAACAGAGGATGGCTTATATTTGCTGG
ACTATAAAACAGACCGGATTGAGGGTAAATTCCAGCACGGCTTTGAAGGAGCAGCCCCGATCCTGAAAAAGAGATATGGA
ACGCAAATTCAGCTGTACACAAGGGCCGTTGAACAAATTGCTCAAACGAAGGTAAAAGGGTGTGCGCTTTATTTCTTTGA
TGGAGGGCACATTCTGACTTTATAA

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-461)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1055-1225)

• UvrD-like helicase C-terminal domain (UvrD_C) (472-881)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	93.112	100	0.931