Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   NDR85_RS05985 Genome accession   NZ_CP098738
Coordinates   1213736..1217236 (+) Length   1166 a.a.
NCBI ID   WP_044156481.1    Uniprot ID   -
Organism   Bacillus halotolerans strain XE48     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1208736..1222236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDR85_RS05965 (NDR85_05965) - 1209591..1210792 (-) 1202 Protein_1110 MDR family MFS transporter -
  NDR85_RS05970 (NDR85_05970) - 1210908..1212638 (+) 1731 WP_044156485.1 ABC transporter substrate-binding protein -
  NDR85_RS05975 (NDR85_05975) - 1212672..1212986 (-) 315 WP_044156484.1 four-helix bundle copper-binding protein -
  NDR85_RS05980 (NDR85_05980) - 1213114..1213551 (-) 438 WP_044156482.1 ferritin family protein -
  NDR85_RS05985 (NDR85_05985) addB 1213736..1217236 (+) 3501 WP_044156481.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NDR85_RS05990 (NDR85_05990) addA 1217223..1220927 (+) 3705 WP_044156480.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NDR85_RS05995 (NDR85_05995) sbcD 1220999..1222177 (+) 1179 WP_044156478.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134754.75 Da        Isoelectric Point: 5.3844

>NTDB_id=697530 NDR85_RS05985 WP_044156481.1 1213736..1217236(+) (addB) [Bacillus halotolerans strain XE48]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDTGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMADSGTASEYRGERVLSE
KLHDLSILYQQMEKSLEDQYLHSEDYLTLLAEHIPQAEDLKGAHVYVDGFYQFTPQEFRVLEQLMLQAEHITFSLTADKP
SHEGEPQELDLFRMTGKTYYRLHQAAKELNLDITYKELDESKRHKRAPELAHLEAQYEARPVIPFAEKQEAFTVMQAANR
RAELEGIAREIHSLVREKGYRYKDMAILARQPEDYKDMVKEVFADYEIPYFIDGKTSMLNHPLVEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLNQPKAKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDEFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEVLYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGEDDI
SLDLFQQMMETGAESLTFSLIPPALDQVFVGNMDLSRIYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLKTIGIELS
SGGRERLLDEHFLIYMALSSPSDRLYVTYPIADAEGKTLLPSIVIKRLEELFPQHRERLLTNEPEQVSDEEQLMYLVNKS
VAQSYTASQLRLWTREYEISDVWWSAYNALMNEPDRLESKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLQLKERQFFKLEAPDIGQLFHSSLKLISDRLREHKIDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSLDWLGMKATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAIRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRLLKAEKDKTVLDWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=697530 NDR85_RS05985 WP_044156481.1 1213736..1217236(+) (addB) [Bacillus halotolerans strain XE48]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGTATTCAAGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATACGGGCGGCATGATTCGTGCTCAAGTCTTCAGCTTTTCACGACTGGCCTGGCGCGTCCTCCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTTACGAGCACGGGCGTACAGATGCTCCTGCGGAAGCTCATTGAAGAGCACAAACAGGAGTT
TAAAGTCTATCAAAAAGCAAGTGATAAAAGCGGTTTTACCGCACAGGTAGAACGGATGCTGACTGAGTTTAAGAGATACT
GTCTGGAACCGGAAGATATTCGCCGGATGGCGGATAGCGGAACAGCTTCCGAGTATCGGGGGGAACGTGTTTTATCTGAA
AAGCTTCATGATTTATCGATCCTGTACCAGCAGATGGAAAAAAGCCTTGAGGATCAGTATCTTCATTCTGAGGATTATTT
GACACTATTGGCAGAGCATATCCCGCAGGCGGAAGATTTGAAAGGGGCACATGTCTATGTAGACGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGTTAGAGCAGCTTATGCTTCAGGCAGAGCATATCACGTTTTCACTCACAGCGGACAAGCCG
TCACATGAGGGGGAGCCGCAGGAACTTGATTTGTTCAGAATGACGGGAAAAACGTATTATCGCTTGCACCAGGCGGCAAA
GGAACTGAATCTGGATATCACCTATAAAGAGCTTGATGAATCAAAGCGGCACAAACGCGCACCGGAATTGGCCCATTTAG
AAGCACAGTATGAAGCGCGTCCGGTTATTCCGTTTGCTGAGAAACAAGAGGCTTTTACTGTGATGCAGGCCGCTAACAGA
CGAGCAGAGCTGGAAGGTATTGCCCGGGAAATCCACAGTCTTGTCAGAGAGAAGGGCTACCGTTACAAGGATATGGCGAT
TCTAGCGCGTCAGCCGGAAGACTATAAGGATATGGTCAAGGAAGTATTTGCAGATTACGAGATTCCTTATTTTATTGACG
GGAAAACTTCTATGCTGAACCACCCGTTAGTAGAATTTATCCGGTCAAGCCTAGACGTATTAAAGGGGAATTGGCGTTAT
GAAGCGGTGTTCCGCTGCGTGAAAACCGAACTGTTATTCCCGCTTAACCAGCCGAAGGCCAAGGTAAGAGAGCAGGTTGA
TCAGCTGGAGAATTACTGTATCGCTTACGGGATCAAAGGCGATCGCTGGACAAAGGGCGATCGTTTCCATTACAGACGTT
TTGTGTCGTTAGATGATGAATTTGCGCAGACCGATCAAGAAATCGAAATGGAAAACATGCTAAATGACACGCGCGATTGG
ATTGTTCCGCCGCTGTTCCAGCTGCAGAAACGAATGAAAAAAGCAAAAACGGTTCAAGAGAAGGCGGAGGTGCTTTACCG
TTATTTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGACAGCGGGCTGAGGAAGACGGCAGAATCATTGAAG
CACAGCAGCACCAGCAGGCGTGGGATGCAGTCATTCAGCTGCTTGAGGAGTTTGTGGAAATGATGGGCGAAGATGACATT
TCTCTCGATTTGTTTCAGCAAATGATGGAGACCGGTGCAGAGTCGCTGACATTCTCTCTCATCCCTCCGGCGCTTGATCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATATACGGCACCTCATGCACCTTTGTGATCGGGGCAAACGACGGCGTCC
TGCCGGCTCGCCCTGACGAAAACGGGGTGCTGTCGGATGATGATCGTGAGTGGCTGAAGACGATAGGTATTGAGCTTTCC
TCCGGCGGGCGGGAGCGTCTGCTTGATGAGCATTTCCTCATCTATATGGCGCTTTCAAGCCCTTCTGACCGCCTTTATGT
GACGTATCCGATTGCTGATGCGGAAGGGAAAACGCTTTTGCCGTCGATTGTAATCAAGCGGCTGGAAGAACTGTTTCCGC
AGCATCGTGAGCGTCTATTGACAAATGAACCGGAGCAGGTCAGTGATGAGGAACAGCTTATGTACCTTGTCAATAAAAGC
GTGGCACAATCATATACCGCTAGCCAGCTCAGATTATGGACGAGGGAATATGAGATCAGTGATGTCTGGTGGAGCGCGTA
CAATGCGCTGATGAATGAACCTGACAGGCTGGAGTCGAAAAAGCTTTTCTCTAGCCTGTTTTTCCGAAATGAAGTCAAGC
AGCTTGAACGCTCTGTATCGAGACAGCTCTATGGGGAACGCATTCAAGGGAGCGTGTCGAGGATGGAAACCTTTAACGCT
TGTCCGTTCTCCCATTTTGCGTCCCACGGGCTGCAGCTGAAGGAACGTCAATTTTTTAAGCTTGAAGCACCGGATATCGG
GCAGCTCTTTCATTCGAGTTTAAAACTGATTTCAGACCGGCTGCGCGAGCACAAAATAGATTGGCGCGACCTAACGAAGG
AGCAGTGCGAGCTGTTTTCTTATGATGCGGTCGAGCGGCTGGCGCCGAAACTGCAAAAAGAAATTCTGCTCAGTTCAAAC
AGACATTATTACGTGAAGGAAAAACTGCAAAAAATTGTGACACGCGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTGCCGATCGGCCTTGAATTGGGCTTTGGCGGAAAGGGCCCGCTCCCGCCGCTTACCTTTAAGCTGAAAAACG
GCTGTACGATGGAGCTCGTCGGGCGGATTGACCGCGTGGATAAAGCGGAAAGCTCTAAAGGCCTGCTCCTCCGAATTGTC
GACTATAAATCCAGCGATAAAGGCCTTGACTTGGCGGAAGTGTATTACGGACTTGCGCTGCAAATGCTGACGTATCTGGA
TCTATCGATTACACATTCGTTAGACTGGCTAGGAATGAAAGCCACGCCGGCCGGAGTGCTGTATTTCCATATTCATGACC
CGATGATACAATCGAATCTTCCGCTCGGACTTGATGAAATTGAACAGGAAATTTTTAAGAAATTTAAGATGAAGGGCCTC
CTCCTCGGTGATCAGGAAGCGATTCGCCTCATGGATACAACCCTTCAAGAAGGAAGATCAAATATCATCAATGCGGGCCT
GAAGAAAGACGGCTCACTCAGATCGGATTCAGCGGCAGTCGGTGAAAAAGAATTTGAGCTGTTGACGAAGCATGTGCGCC
GCACCTTCCAAGAAGCAGGCGAGCAAATCACTGACGGGCGCGTATCCATTGAGCCGTATAAAATGAAGAACAAGACGCCT
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAATCATTAGAAGAAAACGAGTATCGTCTGTTAAAGGCCGA
AAAGGACAAGACGGTACTTGATTGGATAAAGAAGGAGGCTGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(306-668)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

94.254

100

0.943