Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   LC189_RS02355 Genome accession   NZ_CP098727
Coordinates   445007..445504 (+) Length   165 a.a.
NCBI ID   WP_001829055.1    Uniprot ID   -
Organism   Staphylococcus aureus strain SauR3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 440007..450504
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LC189_RS02315 (LC189_002315) - 440051..441037 (+) 987 WP_000161314.1 ROK family glucokinase -
  LC189_RS02320 (LC189_002320) - 441037..441366 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  LC189_RS02325 (LC189_002325) - 441363..441986 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  LC189_RS02330 (LC189_002330) comGA 442038..443012 (+) 975 WP_000697227.1 competence type IV pilus ATPase ComGA Machinery gene
  LC189_RS02335 (LC189_002335) comGB 442984..444054 (+) 1071 WP_000776406.1 competence type IV pilus assembly protein ComGB Machinery gene
  LC189_RS02340 (LC189_002340) comGC 444068..444379 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  LC189_RS02345 (LC189_002345) comGD 444357..444803 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  LC189_RS02350 (LC189_002350) comGE 444790..445089 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  LC189_RS02355 (LC189_002355) comGF 445007..445504 (+) 498 WP_001829055.1 competence type IV pilus minor pilin ComGF Machinery gene
  LC189_RS02360 (LC189_002360) - 445601..445747 (+) 147 WP_001789879.1 hypothetical protein -
  LC189_RS02365 (LC189_002365) - 445737..446261 (+) 525 WP_001015121.1 shikimate kinase -
  LC189_RS02370 (LC189_002370) gcvT 446420..447511 (+) 1092 WP_000093346.1 glycine cleavage system aminomethyltransferase GcvT -
  LC189_RS02375 (LC189_002375) gcvPA 447531..448877 (+) 1347 WP_017804499.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  LC189_RS02380 (LC189_002380) gcvPB 448870..450342 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19183.92 Da        Isoelectric Point: 10.2192

>NTDB_id=697401 LC189_RS02355 WP_001829055.1 445007..445504(+) (comGF) [Staphylococcus aureus strain SauR3]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=697401 LC189_RS02355 WP_001829055.1 445007..445504(+) (comGF) [Staphylococcus aureus strain SauR3]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

98.182

100

0.982