Detailed information NTG0697322

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ND439_RS03345
• Genome accession: NZ_CP098722
• Coordinates: 755074..755865 (-)
• Length: 263 a.a.
• NCBI ID: WP_145497187.1
• Uniprot ID: -
• Organism: Yersinia ruckeri strain NVI-11073
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 750074..760865

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ND439_RS03330 (ND439_03325)	-	751262..752644 (-)	1383	WP_145497176.1	PepSY-associated TM helix domain-containing protein	-
ND439_RS03335 (ND439_03330)	gbpA	752764..754254 (-)	1491	WP_234056998.1	N-acetylglucosamine-binding protein GbpA	-
ND439_RS03340 (ND439_03335)	gspS	754680..755054 (+)	375	WP_004722686.1	type II secretion system pilot lipoprotein GspS	-
ND439_RS03345 (ND439_03340)	pilD	755074..755865 (-)	792	WP_145497187.1	prepilin peptidase	Machinery gene
ND439_RS03350 (ND439_03345)	gspM	755865..756362 (-)	498	WP_230596562.1	type II secretion system protein GspM	-
ND439_RS03355 (ND439_03350)	gspL	756346..757545 (-)	1200	WP_004722681.1	type II secretion system protein GspL	-
ND439_RS03360 (ND439_03355)	gspK	757547..758632 (-)	1086	WP_230596563.1	type II secretion system minor pseudopilin GspK	-
ND439_RS03365 (ND439_03360)	gspJ	758622..759227 (-)	606	WP_071704596.1	type II secretion system minor pseudopilin GspJ	-
ND439_RS03370 (ND439_03365)	gspI	759224..759586 (-)	363	WP_038244547.1	type II secretion system minor pseudopilin GspI	-
ND439_RS03375 (ND439_03370)	-	759583..760137 (-)	555	WP_004722675.1	GspH/FimT family pseudopilin	-
ND439_RS03380 (ND439_03375)	gspG	760182..760649 (-)	468	WP_038244546.1	type II secretion system major pseudopilin GspG	-

Sequence

Protein

• Length: 263 a.a.
• Molecular weight: 29687.88 Da
• Isoelectric Point: 8.2965

>NTDB_id=697322 ND439_RS03345 WP_145497187.1 755074..755865(-) (pilD) [Yersinia ruckeri strain NVI-11073]
MDALYMAQMIAPLWWGGLAVLGLCVGSFLNVAIYRLPLMLTRENPHDFNLCLPHSHCPQCKHSLKWRDNIPVFSWLFLQG
QCRYCRASIPVRYPLVEIMTMLMTLFIAAVIPLGSPLLAALLLSWMLIALTMIDIDHLLLPDNLTLPLLWMGLLFHLFDS
RLALSEAVIGAVAGYLILWCLYWSFWLLTKREALGYGDFKLLAALGAWLGWMALPSLLLIASLCGMSFALAARLLRGRPL
NTALPFGPFLAFGGWILFLQYYR

Nucleotide

• Length: 792 bp

>NTDB_id=697322 ND439_RS03345 WP_145497187.1 755074..755865(-) (pilD) [Yersinia ruckeri strain NVI-11073]
ATGGATGCGCTCTATATGGCTCAAATGATAGCGCCACTGTGGTGGGGCGGGCTGGCGGTTCTTGGCCTGTGTGTCGGTAG
TTTTCTCAACGTCGCTATTTATCGGCTACCGCTGATGTTAACCCGCGAAAATCCGCATGATTTTAATCTGTGTCTACCTC
ATTCCCACTGTCCTCAGTGTAAACATTCGTTGAAATGGCGAGATAATATTCCTGTATTCAGTTGGCTATTCCTGCAAGGA
CAATGTCGTTACTGTCGGGCATCTATCCCAGTGCGCTACCCGCTGGTTGAAATCATGACGATGTTGATGACCTTGTTTAT
TGCTGCAGTCATCCCATTGGGCAGCCCGTTACTGGCCGCACTACTACTCAGTTGGATGCTGATCGCGTTAACCATGATCG
ATATTGACCACTTGCTCTTACCGGATAATCTGACATTGCCTCTGCTATGGATGGGGCTACTTTTTCACCTGTTTGATAGT
CGGTTGGCATTAAGTGAGGCAGTGATTGGTGCTGTCGCAGGATATTTGATCTTATGGTGTCTTTACTGGTCGTTCTGGCT
GTTGACGAAGCGGGAAGCTCTGGGCTACGGAGACTTTAAGTTATTAGCCGCATTAGGTGCCTGGCTAGGATGGATGGCGT
TACCCTCTTTACTTTTGATCGCCTCACTCTGCGGCATGAGTTTCGCACTGGCCGCACGTCTCCTACGGGGCAGACCACTG
AATACGGCACTGCCATTCGGGCCGTTTCTCGCATTCGGTGGCTGGATTCTATTCCTTCAGTACTACCGTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (122-229)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.787	96.578	0.49
pilD	Vibrio campbellii strain DS40M4	46.667	96.958	0.452
pilD	Neisseria gonorrhoeae MS11	44.141	97.338	0.43
pilD	Acinetobacter baumannii D1279779	42.471	98.479	0.418
pilD	Acinetobacter nosocomialis M2	42.085	98.479	0.414