Detailed information NTG0697157

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ND440_RS13050
• Genome accession: NZ_CP098701
• Coordinates: 2838137..2838928 (+)
• Length: 263 a.a.
• NCBI ID: WP_004722684.1
• Uniprot ID: -
• Organism: Yersinia ruckeri strain NVI-5089
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2833137..2843928

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ND440_RS13015 (ND440_13010)	gspG	2833353..2833820 (+)	468	WP_038244546.1	type II secretion system major pseudopilin GspG	-
ND440_RS13020 (ND440_13015)	-	2833865..2834419 (+)	555	WP_248578873.1	GspH/FimT family pseudopilin	-
ND440_RS13025 (ND440_13020)	gspI	2834416..2834778 (+)	363	WP_038244547.1	type II secretion system minor pseudopilin GspI	-
ND440_RS13030 (ND440_13025)	gspJ	2834775..2835380 (+)	606	WP_038251265.1	type II secretion system minor pseudopilin GspJ	-
ND440_RS13035 (ND440_13030)	gspK	2835370..2836455 (+)	1086	WP_230596563.1	type II secretion system minor pseudopilin GspK	-
ND440_RS13040 (ND440_13035)	gspL	2836457..2837656 (+)	1200	WP_004722681.1	type II secretion system protein GspL	-
ND440_RS13045 (ND440_13040)	gspM	2837640..2838137 (+)	498	WP_248578874.1	type II secretion system protein GspM	-
ND440_RS13050 (ND440_13045)	pilD	2838137..2838928 (+)	792	WP_004722684.1	prepilin peptidase	Machinery gene
ND440_RS13055 (ND440_13050)	gspS	2838948..2839322 (-)	375	WP_004722686.1	type II secretion system pilot lipoprotein GspS	-
ND440_RS13060 (ND440_13055)	gbpA	2839748..2841238 (+)	1491	WP_248578875.1	N-acetylglucosamine-binding protein GbpA	-
ND440_RS13065 (ND440_13060)	-	2841358..2842740 (+)	1383	WP_248578876.1	PepSY-associated TM helix domain-containing protein	-

Sequence

Protein

• Length: 263 a.a.
• Molecular weight: 29629.85 Da
• Isoelectric Point: 8.4833

>NTDB_id=697157 ND440_RS13050 WP_004722684.1 2838137..2838928(+) (pilD) [Yersinia ruckeri strain NVI-5089]
MDALYMAQMIAPLWWGGLAVLGLCVGSFLNVAIYRLPLMLTRENPHGFNLCLPHSHCPQCKHSLKWRDNIPVFSWLFLQG
QCRYCRASIPVRYPLVEIMTMLMTLFIAAVIPLGSPLLAALLLSWMLIALTMIDIDHLLLPDNLTLPLLWMGLLFHLFDS
RLALSEAVIGAVAGYLILWCLYWSFWLLTKREALGYGDFKLLAALGAWLGWMALPSLLLIASLCGMSFALAARLLRGRPL
NTALPFGPFLAFGGWILFLQYYR

Nucleotide

• Length: 792 bp

>NTDB_id=697157 ND440_RS13050 WP_004722684.1 2838137..2838928(+) (pilD) [Yersinia ruckeri strain NVI-5089]
ATGGATGCGCTCTATATGGCTCAAATGATAGCGCCACTGTGGTGGGGCGGGCTGGCGGTTCTTGGCCTGTGTGTCGGTAG
TTTTCTCAACGTCGCTATTTATCGGCTACCGCTGATGTTAACCCGCGAAAATCCGCATGGTTTTAATCTGTGTCTACCTC
ATTCCCACTGTCCTCAGTGTAAACATTCGTTGAAATGGCGAGATAATATTCCTGTATTCAGTTGGCTATTCCTGCAAGGA
CAATGTCGTTACTGTCGGGCATCTATCCCAGTGCGCTACCCGCTGGTTGAAATCATGACGATGTTGATGACCTTGTTTAT
TGCTGCAGTCATCCCATTGGGCAGCCCGTTACTGGCCGCACTACTACTCAGTTGGATGCTGATCGCGTTAACCATGATCG
ATATTGACCACTTGCTCTTACCGGATAATCTGACATTGCCTCTGCTATGGATGGGGCTACTTTTTCACCTGTTTGATAGT
CGGTTGGCATTAAGTGAGGCAGTGATTGGTGCTGTCGCAGGATATTTGATCTTATGGTGTCTTTACTGGTCGTTCTGGCT
GTTGACGAAGCGGGAAGCTCTGGGCTACGGAGACTTTAAGTTATTAGCCGCATTAGGTGCCTGGCTAGGATGGATGGCGT
TACCCTCTTTACTTTTGATCGCCTCGCTCTGCGGCATGAGTTTCGCACTGGCCGCACGTCTCCTACGGGGCAGACCACTG
AATACGGCACTGCCATTCGGGCCGTTTCTCGCATTCGGTGGCTGGATTCTATTCCTTCAGTACTACCGTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (122-229)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.181	96.578	0.494
pilD	Vibrio campbellii strain DS40M4	46.667	96.958	0.452
pilD	Neisseria gonorrhoeae MS11	44.531	97.338	0.433
pilD	Acinetobacter baumannii D1279779	42.471	98.479	0.418
pilD	Acinetobacter nosocomialis M2	42.085	98.479	0.414