Detailed information NTG0697125

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ND449_RS03515
• Genome accession: NZ_CP098697
• Coordinates: 780994..781785 (-)
• Length: 263 a.a.
• NCBI ID: WP_096823664.1
• Uniprot ID: -
• Organism: Yersinia ruckeri strain NVI-6614
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 775994..786785

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ND449_RS18120 (ND449_03495)	-	777171..778552 (-)	1382	Protein_687	PepSY-associated TM helix domain-containing protein	-
ND449_RS03505 (ND449_03500)	gbpA	778672..780162 (-)	1491	WP_004722689.1	N-acetylglucosamine-binding protein GbpA	-
ND449_RS03510 (ND449_03505)	gspS	780600..780974 (+)	375	WP_004722686.1	type II secretion system pilot lipoprotein GspS	-
ND449_RS03515 (ND449_03510)	pilD	780994..781785 (-)	792	WP_096823664.1	prepilin peptidase	Machinery gene
ND449_RS03520 (ND449_03515)	gspM	781785..782282 (-)	498	WP_230596562.1	type II secretion system protein GspM	-
ND449_RS03525 (ND449_03520)	gspL	782266..783465 (-)	1200	WP_004722681.1	type II secretion system protein GspL	-
ND449_RS03530 (ND449_03525)	gspK	783467..784552 (-)	1086	WP_234049540.1	type II secretion system minor pseudopilin GspK	-
ND449_RS03535 (ND449_03530)	gspJ	784542..785147 (-)	606	WP_038251265.1	type II secretion system minor pseudopilin GspJ	-
ND449_RS03540 (ND449_03535)	gspI	785144..785506 (-)	363	WP_038244547.1	type II secretion system minor pseudopilin GspI	-
ND449_RS03545 (ND449_03540)	-	785503..786057 (-)	555	WP_004722675.1	GspH/FimT family pseudopilin	-
ND449_RS03550 (ND449_03545)	gspG	786102..786569 (-)	468	WP_096823304.1	type II secretion system major pseudopilin GspG	-

Sequence

Protein

• Length: 263 a.a.
• Molecular weight: 29659.87 Da
• Isoelectric Point: 8.4833

>NTDB_id=697125 ND449_RS03515 WP_096823664.1 780994..781785(-) (pilD) [Yersinia ruckeri strain NVI-6614]
MDALYMAQMIAPLWWSGLAVLGLCVGSFLNVAIYRLPLMLTRENPHGFNLCLPHSHCPQCKHSLKWRDNIPVFSWLFLQG
QCRYCRASIPVRYPLVEIMTMLMTLFIAAVIPLGSPLLAALLLSWMLIALTMIDIDHLLLPDNLTLPLLWMGLLFHLFDS
RLALSEAVIGAVAGYLILWCLYWSFWLLTKREALGYGDFKLLAALGAWLGWMALPSLLLIASLCGMSFALAARLLRGRPL
NTALPFGPFLAFGGWILFLQYYR

Nucleotide

• Length: 792 bp

>NTDB_id=697125 ND449_RS03515 WP_096823664.1 780994..781785(-) (pilD) [Yersinia ruckeri strain NVI-6614]
ATGGATGCGCTCTATATGGCTCAAATGATAGCGCCACTGTGGTGGAGCGGGCTGGCGGTTCTTGGCCTGTGTGTCGGTAG
TTTTCTCAACGTCGCTATTTATCGGCTACCGCTGATGTTAACCCGCGAAAATCCGCATGGTTTTAATCTGTGTCTACCTC
ATTCCCACTGTCCTCAGTGTAAACATTCGTTGAAATGGCGAGATAATATTCCTGTATTCAGTTGGCTATTCCTGCAAGGA
CAATGTCGTTACTGTCGGGCATCTATCCCAGTGCGCTACCCGCTGGTTGAAATCATGACGATGTTGATGACCTTGTTTAT
TGCTGCAGTCATCCCATTGGGCAGCCCGTTACTGGCCGCACTACTACTCAGTTGGATGCTGATCGCGTTAACCATGATCG
ATATTGACCACTTGCTCTTACCGGATAATCTGACATTGCCTCTGCTATGGATGGGGCTACTTTTTCACCTGTTTGATAGT
CGGTTGGCATTAAGTGAGGCAGTGATTGGTGCTGTCGCAGGATATTTGATCTTATGGTGTCTTTACTGGTCGTTCTGGCT
GTTGACGAAGCGGGAAGCTCTGGGCTACGGAGACTTTAAGTTATTAGCCGCATTAGGTGCCTGGCTAGGATGGATGGCGT
TACCCTCTTTACTTTTGATCGCCTCGCTCTGCGGCATGAGTTTCGCACTGGCCGCACGTCTCCTACGGGGCAGACCACTG
AATACGGCACTGCCATTCGGGCCGTTTCTCGCATTCGGTGGCTGGATTCTATTCCTTCAGTACTACCGTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (122-229)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.181	96.578	0.494
pilD	Vibrio campbellii strain DS40M4	46.667	96.958	0.452
pilD	Neisseria gonorrhoeae MS11	44.531	97.338	0.433
pilD	Acinetobacter baumannii D1279779	41.697	100	0.43
pilD	Acinetobacter nosocomialis M2	41.328	100	0.426