Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NEA10_RS20350 Genome accession   NZ_CP098611
Coordinates   4719230..4721245 (-) Length   671 a.a.
NCBI ID   WP_252663174.1    Uniprot ID   -
Organism   Phormidium yuhuli AB48     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4714230..4726245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NEA10_RS20325 (NEA10_20325) - 4714439..4714738 (+) 300 WP_252663169.1 hypothetical protein -
  NEA10_RS20330 (NEA10_20330) msrA 4714950..4715414 (+) 465 WP_252663170.1 peptide-methionine (S)-S-oxide reductase MsrA -
  NEA10_RS20335 (NEA10_20335) - 4715687..4716673 (+) 987 WP_252663171.1 hypothetical protein -
  NEA10_RS20340 (NEA10_20340) - 4716766..4717935 (-) 1170 WP_252663172.1 type II secretion system F family protein -
  NEA10_RS20345 (NEA10_20345) - 4718071..4719189 (-) 1119 WP_252663173.1 type IV pilus twitching motility protein PilT -
  NEA10_RS20350 (NEA10_20350) pilB/pilB1 4719230..4721245 (-) 2016 WP_252663174.1 GspE/PulE family protein Machinery gene
  NEA10_RS20355 (NEA10_20355) grpE 4721631..4722401 (+) 771 WP_252663175.1 nucleotide exchange factor GrpE -
  NEA10_RS20360 (NEA10_20360) dnaK 4722490..4724775 (+) 2286 WP_252663176.1 molecular chaperone DnaK -
  NEA10_RS20365 (NEA10_20365) dnaJ 4724874..4725998 (+) 1125 WP_252663177.1 molecular chaperone DnaJ -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 74935.20 Da        Isoelectric Point: 4.7203

>NTDB_id=697040 NEA10_RS20350 WP_252663174.1 4719230..4721245(-) (pilB/pilB1) [Phormidium yuhuli AB48]
MTNFSASAPKARRALVVQTNFSPFGNKLIQAGHVSPDQMSQAQEDSRLSGRPLIEAIESITGKQLPPELIRQYKKQQLFE
LKILYGVEAFDPEVTELPPGQVNKLLEFVSVDSCRRYRFVPLELKENAVVVAMVDPDNLEAQDDLKRILRAKNYGLQRQA
ITPDDYQSLISVYLDEQVKKEAESQKREQAEKNAITVEDVAQLAGEDDDLEEAPPEAADLEEEADAEDKSVIALVNKILR
KALTDDVSDIHVEPQEEFLRIRMRKDGVLHEPFPRLPKLIIPAITSRFKIISDMDIAERRQAQDGRIRRMFQGRAVDFRV
NALPTRYGEKIVLRILDNSSTQLGLDKLISDPETLEKVREVSSRPFGLILVTGPTGSGKSTTLYSILAERNDPGVNISTA
EDPIEYALDGISQCQVIREKDLTFANILRAFLRQDPDIILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLD
EMGVEPFMISGSLLGVLAQRLMRRVCSECRIPYTPTREELARYGMSASSDVDATFYKANTVPPGDRQELQERGELCPKCG
GVGYKGRVGVYEFLQTTEELALLINENAPTERIKEKAVEQGMTTLLAYSLNLVKQGHTTFEEVDRVTFTDSGLEAELKAK
RKSSLTCNNCGAGLDPEWMDCPYCMTPRFQD

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=697040 NEA10_RS20350 WP_252663174.1 4719230..4721245(-) (pilB/pilB1) [Phormidium yuhuli AB48]
ATGACTAACTTTTCTGCTAGTGCCCCCAAAGCTCGGCGTGCCTTGGTGGTTCAGACGAACTTTTCTCCCTTTGGAAATAA
ACTCATTCAAGCGGGTCACGTCAGCCCGGATCAGATGAGCCAGGCCCAAGAAGATAGCCGCCTGTCGGGTCGTCCCTTGA
TTGAGGCAATTGAAAGCATTACTGGAAAACAACTGCCCCCCGAGTTAATCCGCCAGTATAAAAAGCAACAACTGTTTGAG
TTAAAGATTCTTTACGGGGTTGAGGCGTTCGATCCAGAGGTGACGGAGTTACCGCCGGGGCAAGTGAATAAGCTCTTGGA
ATTTGTCTCGGTGGATAGTTGTCGTCGCTACCGCTTTGTTCCCTTGGAATTGAAGGAAAATGCGGTGGTGGTGGCCATGG
TGGACCCGGACAACCTAGAGGCTCAGGACGACCTGAAGCGGATTCTGCGGGCTAAAAACTACGGTTTGCAACGGCAGGCG
ATTACCCCGGATGACTATCAATCTCTGATTTCGGTTTACCTGGATGAACAGGTGAAAAAGGAAGCCGAAAGCCAAAAACG
GGAACAGGCTGAAAAGAATGCCATTACCGTTGAAGATGTGGCTCAGTTGGCCGGAGAGGATGATGACTTGGAAGAAGCCC
CCCCAGAAGCGGCGGATTTGGAGGAGGAGGCAGATGCTGAGGACAAAAGCGTCATTGCTCTGGTTAATAAGATTCTCCGT
AAAGCTCTGACGGATGATGTCTCTGATATCCATGTCGAGCCGCAAGAAGAGTTTCTCCGGATTCGGATGCGTAAGGATGG
GGTGCTCCATGAGCCGTTTCCTCGCCTTCCCAAGTTGATTATTCCAGCAATTACCTCCCGTTTTAAGATTATTTCGGATA
TGGATATTGCTGAACGCCGTCAAGCCCAGGATGGACGGATTAGACGGATGTTCCAAGGCCGAGCGGTGGATTTCCGGGTG
AATGCCCTCCCCACCCGCTATGGTGAGAAAATTGTCTTGCGGATTCTGGATAACTCTTCGACTCAGTTGGGGTTGGATAA
ACTCATCTCTGATCCCGAAACCCTGGAAAAAGTGCGGGAAGTCTCCAGTCGTCCCTTCGGGTTGATTTTGGTGACGGGGC
CGACGGGGTCTGGGAAGTCAACGACCCTCTATTCGATTTTGGCAGAACGGAATGACCCGGGGGTCAATATTAGTACGGCG
GAAGATCCCATTGAGTATGCTCTCGATGGGATTAGTCAATGTCAGGTGATTCGGGAAAAAGACTTGACCTTTGCCAACAT
CCTGCGGGCGTTTCTGCGGCAAGACCCGGATATTATTCTGGTGGGTGAGACGCGAGATAAGGAAACGGCAAAAACGGCAA
TTGAGGCCGCGTTGACGGGTCACTTGGTCTTGACGACGCTGCACACCAACGATGCGGCGGGGGCGATCGCTCGTCTGGAT
GAGATGGGGGTTGAACCCTTCATGATTTCTGGCTCCCTGTTGGGAGTATTGGCCCAACGGCTGATGCGGCGGGTTTGTTC
GGAATGTCGCATTCCCTACACTCCAACTCGCGAGGAGTTGGCTCGCTATGGGATGAGTGCTTCGAGTGATGTGGATGCAA
CGTTCTATAAGGCCAACACGGTTCCGCCGGGCGATCGCCAAGAGTTACAAGAGCGCGGCGAGCTATGTCCTAAATGTGGT
GGAGTTGGCTATAAAGGACGGGTGGGGGTCTATGAGTTCCTACAAACCACTGAGGAACTGGCTTTGCTGATTAATGAAAA
TGCACCCACGGAACGGATTAAGGAGAAAGCCGTTGAGCAGGGGATGACGACGTTGTTGGCTTATAGTCTCAATTTGGTGA
AACAGGGGCATACTACCTTTGAGGAGGTGGATCGGGTCACGTTCACAGACTCTGGGTTAGAGGCTGAACTGAAAGCTAAA
CGCAAGAGTTCGCTCACCTGTAACAACTGTGGAGCAGGCTTGGATCCTGAGTGGATGGATTGTCCCTACTGTATGACCCC
CCGTTTCCAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(237-504)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(74-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

62.689

98.659

0.618
  pilF Thermus thermophilus HB27

40.843

91.952

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.839

92.399

0.368