Detailed information NTG0695495

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NCW68_RS21430
• Genome accession: NZ_CP098427
• Coordinates: 4372769..4373569 (+)
• Length: 266 a.a.
• NCBI ID: WP_004191960.1
• Uniprot ID: A0AAJ5B1F9
• Organism: Klebsiella pneumoniae strain 5844
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4367769..4378569

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NCW68_RS21395 (NCW68_21340)	gspH	4367925..4368440 (+)	516	WP_032422016.1	type II secretion system minor pseudopilin GspH	-
NCW68_RS21400 (NCW68_21345)	gspI	4368437..4368802 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
NCW68_RS21405 (NCW68_21350)	gspJ	4368799..4369395 (+)	597	WP_032433846.1	type II secretion system minor pseudopilin GspJ	-
NCW68_RS21410 (NCW68_21355)	gspK	4369392..4370378 (+)	987	WP_077255453.1	type II secretion system minor pseudopilin GspK	-
NCW68_RS21415 (NCW68_21360)	gspL	4370365..4371558 (+)	1194	WP_032416685.1	type II secretion system protein GspL	-
NCW68_RS21420 (NCW68_21365)	-	4371551..4372036 (+)	486	WP_004145896.1	type II secretion system protein M	-
NCW68_RS21425 (NCW68_21370)	-	4372026..4372772 (+)	747	WP_004145897.1	type II secretion system protein N	-
NCW68_RS21430 (NCW68_21375)	pilD	4372769..4373569 (+)	801	WP_004191960.1	prepilin peptidase	Machinery gene
NCW68_RS21435 (NCW68_21380)	hrpB	4373617..4376046 (-)	2430	WP_064472269.1	ATP-dependent helicase HrpB	-
NCW68_RS21440 (NCW68_21385)	thpR	4376119..4376655 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
NCW68_RS21445 (NCW68_21390)	sfsA	4376655..4377371 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
NCW68_RS21450 (NCW68_21395)	dksA	4377534..4377989 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29403.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=695495 NCW68_RS21430 WP_004191960.1 4372769..4373569(+) (pilD) [Klebsiella pneumoniae strain 5844]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=695495 NCW68_RS21430 WP_004191960.1 4372769..4373569(+) (pilD) [Klebsiella pneumoniae strain 5844]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421