Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NCL52_RS12390 Genome accession   NZ_CP098417
Coordinates   2424922..2425992 (-) Length   356 a.a.
NCBI ID   WP_128738022.1    Uniprot ID   -
Organism   Bacillus subtilis strain N2-10     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2419922..2430992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NCL52_RS12345 (NCL52_12345) tapA 2420400..2421161 (-) 762 WP_101169542.1 amyloid fiber anchoring/assembly protein TapA -
  NCL52_RS12350 (NCL52_12350) yqzG 2421434..2421760 (+) 327 WP_024573388.1 YqzG/YhdC family protein -
  NCL52_RS12355 (NCL52_12355) spoIITA 2421802..2421981 (-) 180 WP_014480252.1 YqzE family protein -
  NCL52_RS12360 (NCL52_12360) comGG 2422052..2422426 (-) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  NCL52_RS12365 (NCL52_12365) comGF 2422427..2422810 (-) 384 WP_128738019.1 ComG operon protein ComGF Machinery gene
  NCL52_RS12370 (NCL52_12370) comGE 2422836..2423183 (-) 348 WP_128738020.1 ComG operon protein 5 Machinery gene
  NCL52_RS12375 (NCL52_12375) comGD 2423167..2423598 (-) 432 WP_024573390.1 comG operon protein ComGD Machinery gene
  NCL52_RS12380 (NCL52_12380) comGC 2423588..2423884 (-) 297 WP_024573391.1 comG operon protein ComGC Machinery gene
  NCL52_RS12385 (NCL52_12385) comGB 2423898..2424935 (-) 1038 WP_128738021.1 comG operon protein ComGB Machinery gene
  NCL52_RS12390 (NCL52_12390) comGA 2424922..2425992 (-) 1071 WP_128738022.1 competence protein ComGA Machinery gene
  NCL52_RS20750 - 2426203..2426328 (-) 126 WP_003230155.1 hypothetical protein -
  NCL52_RS12395 (NCL52_12395) corA 2426394..2427347 (-) 954 WP_004399136.1 magnesium transporter CorA -
  NCL52_RS12400 (NCL52_12400) yqhB 2427490..2428818 (+) 1329 WP_003230150.1 hemolysin family protein -
  NCL52_RS12405 (NCL52_12405) rsbRD 2428871..2429707 (-) 837 WP_015384093.1 RsbT co-antagonist protein RsbRD -
  NCL52_RS12415 (NCL52_12415) mgsR 2429931..2430311 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  NCL52_RS12420 (NCL52_12420) yqgY 2430543..2430788 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40444.62 Da        Isoelectric Point: 9.0175

>NTDB_id=695390 NCL52_RS12390 WP_128738022.1 2424922..2425992(-) (comGA) [Bacillus subtilis strain N2-10]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAVIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=695390 NCL52_RS12390 WP_128738022.1 2424922..2425992(-) (comGA) [Bacillus subtilis strain N2-10]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATCGTGCC
GAGGGAGCGGGACGCTGTCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCCACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGCCAGTCAC
GAAATACCAGGAGAGCTAGTGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.719

100

0.997