Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NCL52_RS02000 Genome accession   NZ_CP098417
Coordinates   372292..372741 (-) Length   149 a.a.
NCBI ID   WP_024572180.1    Uniprot ID   -
Organism   Bacillus subtilis strain N2-10     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 367292..377741
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NCL52_RS01960 (NCL52_01960) - 367613..367810 (+) 198 WP_134982414.1 hypothetical protein -
  NCL52_RS01965 (NCL52_01965) yckC 367830..368285 (+) 456 WP_137200561.1 RDD family protein -
  NCL52_RS01970 (NCL52_01970) yckD 368367..368699 (+) 333 WP_137200563.1 YckD family protein -
  NCL52_RS01975 (NCL52_01975) - 368827..369249 (+) 423 WP_175405006.1 MarR family transcriptional regulator -
  NCL52_RS01980 (NCL52_01980) - 369237..369578 (+) 342 WP_032724911.1 DUF3147 family protein -
  NCL52_RS01985 (NCL52_01985) - 369597..370325 (+) 729 WP_021481826.1 AAA family ATPase -
  NCL52_RS01990 (NCL52_01990) bglC 370397..371830 (+) 1434 WP_134982418.1 6-phospho-beta-glucosidase -
  NCL52_RS01995 (NCL52_01995) nin/comJ 371867..372265 (-) 399 WP_015252845.1 DNA-entry nuclease inhibitor Regulator
  NCL52_RS02000 (NCL52_02000) nucA/comI 372292..372741 (-) 450 WP_024572180.1 DNA-entry nuclease Machinery gene
  NCL52_RS02005 (NCL52_02005) tlpC 372909..374633 (-) 1725 WP_137200565.1 methyl-accepting chemotaxis protein TlpC -
  NCL52_RS02010 (NCL52_02010) hxlB 374744..375301 (-) 558 WP_134982420.1 6-phospho-3-hexuloisomerase -
  NCL52_RS02015 (NCL52_02015) hxlA 375307..375939 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  NCL52_RS02020 (NCL52_02020) hxlR 376171..376533 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16428.34 Da        Isoelectric Point: 4.5282

>NTDB_id=695348 NCL52_RS02000 WP_024572180.1 372292..372741(-) (nucA/comI) [Bacillus subtilis strain N2-10]
MNITTDIIKTILLVIVIIAAAAAGLIKGDFFSADQKTSQTKEYDETISFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=695348 NCL52_RS02000 WP_024572180.1 372292..372741(-) (nucA/comI) [Bacillus subtilis strain N2-10]
GTGAACATCACGACGGACATCATAAAAACAATACTTCTCGTCATCGTCATAATAGCAGCTGCAGCTGCCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCTCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.315

100

0.973