Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   NB631_RS13245 Genome accession   NZ_CP098257
Coordinates   2710996..2711493 (+) Length   165 a.a.
NCBI ID   WP_001794328.1    Uniprot ID   -
Organism   Staphylococcus aureus strain M4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2705996..2716493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NB631_RS13205 (NB631_13215) - 2706040..2707026 (+) 987 WP_000161314.1 ROK family glucokinase -
  NB631_RS13210 (NB631_13220) - 2707026..2707355 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  NB631_RS13215 (NB631_13225) - 2707352..2707975 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  NB631_RS13220 (NB631_13230) comGA 2708027..2709001 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  NB631_RS13225 (NB631_13235) comGB 2708973..2710043 (+) 1071 WP_000776412.1 competence type IV pilus assembly protein ComGB Machinery gene
  NB631_RS13230 (NB631_13240) comGC 2710057..2710368 (+) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  NB631_RS13235 (NB631_13245) comGD 2710346..2710792 (+) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  NB631_RS13240 (NB631_13250) comGE 2710779..2711078 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  NB631_RS13245 (NB631_13255) comGF 2710996..2711493 (+) 498 WP_001794328.1 competence type IV pilus minor pilin ComGF Machinery gene
  NB631_RS13250 (NB631_13260) - 2711590..2711736 (+) 147 WP_001789879.1 hypothetical protein -
  NB631_RS13255 (NB631_13265) - 2711726..2712250 (+) 525 WP_001015125.1 shikimate kinase -
  NB631_RS13260 (NB631_13270) gcvT 2712409..2713500 (+) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  NB631_RS13265 (NB631_13275) gcvPA 2713520..2714866 (+) 1347 WP_000019697.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  NB631_RS13270 (NB631_13280) gcvPB 2714859..2716331 (+) 1473 WP_000202178.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19197.95 Da        Isoelectric Point: 10.2192

>NTDB_id=694865 NB631_RS13245 WP_001794328.1 2710996..2711493(+) (comGF) [Staphylococcus aureus strain M4]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=694865 NB631_RS13245 WP_001794328.1 2710996..2711493(+) (comGF) [Staphylococcus aureus strain M4]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.788

100

0.988
  comGF Staphylococcus aureus MW2

97.576

100

0.976