Detailed information NTG0694017

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NBY42_RS04480
• Genome accession: NZ_CP098154
• Coordinates: 947302..948102 (-)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain Z0117KP0017
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 942302..953102

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NBY42_RS04460 (NBY42_04460)	dksA	942882..943337 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
NBY42_RS04465 (NBY42_04465)	sfsA	943500..944216 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
NBY42_RS04470 (NBY42_04470)	thpR	944216..944752 (-)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
NBY42_RS04475 (NBY42_04475)	hrpB	944825..947254 (+)	2430	WP_075253266.1	ATP-dependent helicase HrpB	-
NBY42_RS04480 (NBY42_04480)	pilD	947302..948102 (-)	801	WP_004145898.1	prepilin peptidase	Machinery gene
NBY42_RS04485 (NBY42_04485)	-	948099..948845 (-)	747	WP_057222205.1	type II secretion system protein N	-
NBY42_RS04490 (NBY42_04490)	-	948835..949320 (-)	486	WP_057222206.1	type II secretion system protein M	-
NBY42_RS04495 (NBY42_04495)	gspL	949313..950506 (-)	1194	WP_075253264.1	type II secretion system protein GspL	-
NBY42_RS04500 (NBY42_04500)	gspK	950493..951479 (-)	987	WP_094326285.1	type II secretion system minor pseudopilin GspK	-
NBY42_RS04505 (NBY42_04505)	gspJ	951476..952072 (-)	597	WP_023342989.1	type II secretion system minor pseudopilin GspJ	-
NBY42_RS04510 (NBY42_04510)	gspI	952069..952434 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
NBY42_RS04515 (NBY42_04515)	gspH	952431..952946 (-)	516	WP_057222208.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=694017 NBY42_RS04480 WP_004145898.1 947302..948102(-) (pilD) [Klebsiella pneumoniae strain Z0117KP0017]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=694017 NBY42_RS04480 WP_004145898.1 947302..948102(-) (pilD) [Klebsiella pneumoniae strain Z0117KP0017]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGGCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425