Detailed information NTG0692766

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M9O80_RS21680
• Genome accession: NZ_CP097808
• Coordinates: 4459682..4460482 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola strain 17YN56
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4454682..4465482

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M9O80_RS21645 (M9O80_21645)	gspH	4454835..4455350 (+)	516	WP_250294536.1	type II secretion system minor pseudopilin GspH	-
M9O80_RS21650 (M9O80_21650)	gspI	4455347..4455712 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
M9O80_RS21655 (M9O80_21655)	gspJ	4455709..4456305 (+)	597	WP_064171240.1	type II secretion system minor pseudopilin GspJ	-
M9O80_RS21660 (M9O80_21660)	gspK	4456302..4457282 (+)	981	WP_077267012.1	type II secretion system minor pseudopilin GspK	-
M9O80_RS21665 (M9O80_21665)	gspL	4457275..4458471 (+)	1197	WP_064171241.1	type II secretion system protein GspL	-
M9O80_RS21670 (M9O80_21670)	-	4458464..4458949 (+)	486	WP_012968921.1	type II secretion system protein M	-
M9O80_RS21675 (M9O80_21675)	-	4458939..4459685 (+)	747	WP_032740433.1	type II secretion system protein N	-
M9O80_RS21680 (M9O80_21680)	pilD	4459682..4460482 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
M9O80_RS21685 (M9O80_21685)	hrpB	4460529..4462958 (-)	2430	WP_110221779.1	ATP-dependent helicase HrpB	-
M9O80_RS21690 (M9O80_21690)	thpR	4463031..4463567 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
M9O80_RS21695 (M9O80_21695)	sfsA	4463567..4464283 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
M9O80_RS21700 (M9O80_21700)	dksA	4464446..4464901 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=692766 M9O80_RS21680 WP_008807384.1 4459682..4460482(+) (pilD) [Klebsiella variicola strain 17YN56]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=692766 M9O80_RS21680 WP_008807384.1 4459682..4460482(+) (pilD) [Klebsiella variicola strain 17YN56]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425