Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M9420_RS05735 Genome accession   NZ_CP097796
Coordinates   1191849..1192394 (+) Length   181 a.a.
NCBI ID   WP_014391406.1    Uniprot ID   -
Organism   Pasteurella multocida strain 40540     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1186849..1197394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9420_RS05710 (M9420_05710) - 1187276..1188205 (+) 930 WP_014391409.1 phosphatidate cytidylyltransferase -
  M9420_RS05715 (M9420_05715) mog 1188231..1188809 (+) 579 WP_014391408.1 molybdopterin adenylyltransferase -
  M9420_RS05720 (M9420_05720) glnB 1188854..1189192 (+) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  M9420_RS05725 (M9420_05725) - 1189193..1190446 (+) 1254 WP_014391407.1 AI-2E family transporter -
  M9420_RS05730 (M9420_05730) - 1190626..1191810 (+) 1185 WP_014668288.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M9420_RS05735 (M9420_05735) pilF/pilF2 1191849..1192394 (+) 546 WP_014391406.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M9420_RS05740 (M9420_05740) - 1192517..1193473 (+) 957 WP_014391405.1 RodZ family helix-turn-helix domain-containing protein -
  M9420_RS05745 (M9420_05745) ispG 1193500..1194603 (+) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M9420_RS05750 (M9420_05750) hisS 1194633..1195904 (+) 1272 WP_010907448.1 histidine--tRNA ligase -
  M9420_RS05755 (M9420_05755) - 1195923..1196534 (+) 612 WP_005725137.1 YfgM family protein -
  M9420_RS05760 (M9420_05760) - 1196747..1197184 (+) 438 WP_010907449.1 isoprenylcysteine carboxylmethyltransferase family protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20884.46 Da        Isoelectric Point: 8.1419

>NTDB_id=692623 M9420_RS05735 WP_014391406.1 1191849..1192394(+) (pilF/pilF2) [Pasteurella multocida strain 40540]
MNRTFRFGIASIFSLLLCACVSQESNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPSRAKKFPPYIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=692623 M9420_RS05735 WP_014391406.1 1191849..1192394(+) (pilF/pilF2) [Pasteurella multocida strain 40540]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTGCTTTGTGCTTGTGTGAGTCAAGAAAGTAACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCTGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGCCAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAGTAGAGCAAAAAAATTTCCGCCATATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.695

92.265

0.486
  pilF Glaesserella parasuis strain SC1401

45.946

100

0.47