Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M9414_RS02125 Genome accession   NZ_CP097795
Coordinates   443535..444080 (+) Length   181 a.a.
NCBI ID   WP_083004641.1    Uniprot ID   -
Organism   Pasteurella multocida strain 34030     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 438535..449080
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9414_RS02100 (M9414_02100) - 438960..439889 (+) 930 WP_059245741.1 phosphatidate cytidylyltransferase -
  M9414_RS02105 (M9414_02105) mog 439915..440493 (+) 579 WP_059245742.1 molybdopterin adenylyltransferase -
  M9414_RS02110 (M9414_02110) glnB 440538..440876 (+) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  M9414_RS02115 (M9414_02115) - 440877..442130 (+) 1254 WP_005755688.1 AI-2E family transporter -
  M9414_RS02120 (M9414_02120) - 442311..443495 (+) 1185 WP_250046932.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M9414_RS02125 (M9414_02125) pilF/pilF2 443535..444080 (+) 546 WP_083004641.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M9414_RS02130 (M9414_02130) - 444203..445159 (+) 957 WP_250046933.1 RodZ family helix-turn-helix domain-containing protein -
  M9414_RS02135 (M9414_02135) ispG 445186..446289 (+) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M9414_RS02140 (M9414_02140) hisS 446319..447590 (+) 1272 WP_250046934.1 histidine--tRNA ligase -
  M9414_RS02145 (M9414_02145) - 447609..448220 (+) 612 WP_083004649.1 YfgM family protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20819.37 Da        Isoelectric Point: 7.9056

>NTDB_id=692593 M9414_RS02125 WP_083004641.1 443535..444080(+) (pilF/pilF2) [Pasteurella multocida strain 34030]
MTRVFQLGIASIFSLFLSACVSQEGHKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=692593 M9414_RS02125 WP_083004641.1 443535..444080(+) (pilF/pilF2) [Pasteurella multocida strain 34030]
ATGACGCGCGTTTTTCAATTAGGGATAGCAAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTCACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGTGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGCCAAAGATGCTGTCCGTTATCAACAA
AATTTGCAACACTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.695

92.265

0.486
  pilF Glaesserella parasuis strain SC1401

44.385

100

0.459