Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M9413_RS02420 Genome accession   NZ_CP097794
Coordinates   505813..506358 (+) Length   181 a.a.
NCBI ID   WP_250265482.1    Uniprot ID   -
Organism   Pasteurella multocida strain 28606     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 500813..511358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9413_RS02405 (M9413_02405) - 502140..502640 (+) 501 WP_250023345.1 tetratricopeptide repeat protein -
  M9413_RS02410 (M9413_02410) - 502811..504409 (+) 1599 WP_250023342.1 tetratricopeptide repeat protein -
  M9413_RS02415 (M9413_02415) - 504589..505773 (+) 1185 WP_284346603.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M9413_RS02420 (M9413_02420) pilF/pilF2 505813..506358 (+) 546 WP_250265482.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M9413_RS02425 (M9413_02425) - 506481..507437 (+) 957 WP_250265483.1 RodZ family helix-turn-helix domain-containing protein -
  M9413_RS02430 (M9413_02430) ispG 507464..508567 (+) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M9413_RS02435 (M9413_02435) hisS 508597..509868 (+) 1272 WP_005755693.1 histidine--tRNA ligase -
  M9413_RS02440 (M9413_02440) - 509887..510498 (+) 612 WP_005725137.1 YfgM family protein -
  M9413_RS02445 (M9413_02445) - 510707..511144 (+) 438 WP_250265590.1 isoprenylcysteine carboxylmethyltransferase family protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20871.41 Da        Isoelectric Point: 7.8913

>NTDB_id=692574 M9413_RS02420 WP_250265482.1 505813..506358(+) (pilF/pilF2) [Pasteurella multocida strain 28606]
MTRVFQVWIASIFSLFLSACVSQEGNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHASNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGAFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=692574 M9413_RS02420 WP_250265482.1 505813..506358(+) (pilF/pilF2) [Pasteurella multocida strain 28606]
ATGACGCGCGTTTTTCAAGTATGGATAGCAAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTAACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCTCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTGCCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

50

97.238

0.486
  pilF Glaesserella parasuis strain SC1401

43.85

100

0.453