Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M9417_RS06280 Genome accession   NZ_CP097793
Coordinates   1249846..1250391 (+) Length   181 a.a.
NCBI ID   WP_014391406.1    Uniprot ID   -
Organism   Pasteurella multocida strain 21275     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1244846..1255391
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9417_RS06255 (M9417_06255) - 1245273..1246202 (+) 930 WP_014391409.1 phosphatidate cytidylyltransferase -
  M9417_RS06260 (M9417_06260) mog 1246228..1246806 (+) 579 WP_014391408.1 molybdopterin adenylyltransferase -
  M9417_RS06265 (M9417_06265) glnB 1246851..1247189 (+) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  M9417_RS06270 (M9417_06270) - 1247190..1248443 (+) 1254 WP_014391407.1 AI-2E family transporter -
  M9417_RS06275 (M9417_06275) - 1248623..1249807 (+) 1185 WP_005752447.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M9417_RS06280 (M9417_06280) pilF/pilF2 1249846..1250391 (+) 546 WP_014391406.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M9417_RS06285 (M9417_06285) - 1250514..1251470 (+) 957 WP_014391405.1 RodZ family helix-turn-helix domain-containing protein -
  M9417_RS06290 (M9417_06290) ispG 1251497..1252600 (+) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M9417_RS06295 (M9417_06295) hisS 1252630..1253901 (+) 1272 WP_041423113.1 histidine--tRNA ligase -
  M9417_RS06300 (M9417_06300) - 1253920..1254531 (+) 612 WP_005725137.1 YfgM family protein -
  M9417_RS06305 (M9417_06305) - 1254740..1255177 (+) 438 WP_005758199.1 isoprenylcysteine carboxylmethyltransferase family protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20884.46 Da        Isoelectric Point: 8.1419

>NTDB_id=692559 M9417_RS06280 WP_014391406.1 1249846..1250391(+) (pilF/pilF2) [Pasteurella multocida strain 21275]
MNRTFRFGIASIFSLLLCACVSQESNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPSRAKKFPPYIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=692559 M9417_RS06280 WP_014391406.1 1249846..1250391(+) (pilF/pilF2) [Pasteurella multocida strain 21275]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTGCTTTGTGCTTGTGTGAGTCAAGAAAGTAACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCTGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGCCAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAGTAGAGCAAAAAAATTTCCGCCATATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.695

92.265

0.486
  pilF Glaesserella parasuis strain SC1401

45.946

100

0.47