Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M9422_RS07325 Genome accession   NZ_CP097792
Coordinates   1576186..1576731 (-) Length   181 a.a.
NCBI ID   WP_010907447.1    Uniprot ID   -
Organism   Pasteurella multocida strain 36502     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1571186..1581731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9422_RS07300 (M9422_07300) - 1571405..1571842 (-) 438 WP_010907449.1 isoprenylcysteine carboxylmethyltransferase family protein -
  M9422_RS07305 (M9422_07305) - 1572047..1572657 (-) 611 Protein_1401 YfgM family protein -
  M9422_RS07310 (M9422_07310) hisS 1572676..1573947 (-) 1272 WP_010907448.1 histidine--tRNA ligase -
  M9422_RS07315 (M9422_07315) ispG 1573977..1575080 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M9422_RS07320 (M9422_07320) - 1575107..1576063 (-) 957 WP_005725132.1 RodZ family helix-turn-helix domain-containing protein -
  M9422_RS07325 (M9422_07325) pilF/pilF2 1576186..1576731 (-) 546 WP_010907447.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M9422_RS07330 (M9422_07330) - 1576770..1577954 (-) 1185 WP_010907446.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M9422_RS07335 (M9422_07335) - 1578134..1579549 (-) 1416 WP_250245403.1 tetratricopeptide repeat protein -
  M9422_RS07340 (M9422_07340) - 1579567..1580499 (-) 933 WP_250245404.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20911.47 Da        Isoelectric Point: 7.8913

>NTDB_id=692529 M9422_RS07325 WP_010907447.1 1576186..1576731(-) (pilF/pilF2) [Pasteurella multocida strain 36502]
MTRVFQVWIASIFSLFLSACVSQEGNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGAFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDTVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=692529 M9422_RS07325 WP_010907447.1 1576186..1576731(-) (pilF/pilF2) [Pasteurella multocida strain 36502]
ATGACGCGCGTTTTTCAAGTATGGATAGCAAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTAACAA
AACGTTTGATTCACATGAGGCAGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTGCCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGACACTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

50

97.238

0.486
  pilF Glaesserella parasuis strain SC1401

43.85

100

0.453