Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M9415_RS03385 Genome accession   NZ_CP097790
Coordinates   693776..694321 (+) Length   181 a.a.
NCBI ID   WP_046333479.1    Uniprot ID   -
Organism   Pasteurella multocida strain 31971     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 688776..699321
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9415_RS03360 (M9415_03360) - 689202..690131 (+) 930 WP_005725108.1 phosphatidate cytidylyltransferase -
  M9415_RS03365 (M9415_03365) mog 690157..690735 (+) 579 WP_046333482.1 molybdopterin adenylyltransferase -
  M9415_RS03370 (M9415_03370) glnB 690780..691118 (+) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  M9415_RS03375 (M9415_03375) - 691119..692372 (+) 1254 WP_005755688.1 AI-2E family transporter -
  M9415_RS03380 (M9415_03380) - 692553..693737 (+) 1185 WP_046333480.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M9415_RS03385 (M9415_03385) pilF/pilF2 693776..694321 (+) 546 WP_046333479.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M9415_RS03390 (M9415_03390) - 694444..695400 (+) 957 WP_046333478.1 RodZ family helix-turn-helix domain-containing protein -
  M9415_RS03395 (M9415_03395) ispG 695427..696530 (+) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M9415_RS03400 (M9415_03400) hisS 696560..697831 (+) 1272 WP_046333477.1 histidine--tRNA ligase -
  M9415_RS03405 (M9415_03405) - 697850..698461 (+) 612 WP_046333474.1 YfgM family protein -
  M9415_RS03410 (M9415_03410) - 698673..699110 (+) 438 WP_010907449.1 isoprenylcysteine carboxylmethyltransferase family protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20829.28 Da        Isoelectric Point: 7.8917

>NTDB_id=692488 M9415_RS03385 WP_046333479.1 693776..694321(+) (pilF/pilF2) [Pasteurella multocida strain 31971]
MNRTFRFGIASIFSLFLSACVSQEGNNTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGAFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=692488 M9415_RS03385 WP_046333479.1 693776..694321(+) (pilF/pilF2) [Pasteurella multocida strain 31971]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTAACAA
TACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTGCCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.096

92.265

0.481
  pilF Glaesserella parasuis strain SC1401

44.865

100

0.459