Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M9412_RS08090 Genome accession   NZ_CP097789
Coordinates   1697099..1697644 (+) Length   181 a.a.
NCBI ID   WP_250262399.1    Uniprot ID   -
Organism   Pasteurella multocida strain 35564     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1692099..1702644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9412_RS08065 (M9412_08065) glnB 1692375..1692713 (+) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  M9412_RS08070 (M9412_08070) - 1692714..1693967 (+) 1254 WP_250262396.1 AI-2E family transporter -
  M9412_RS08075 (M9412_08075) - 1694085..1694327 (+) 243 WP_250262397.1 hypothetical protein -
  M9412_RS08080 (M9412_08080) - 1694300..1695694 (+) 1395 WP_250262398.1 tetratricopeptide repeat protein -
  M9412_RS08085 (M9412_08085) - 1695875..1697059 (+) 1185 WP_005752447.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M9412_RS08090 (M9412_08090) pilF/pilF2 1697099..1697644 (+) 546 WP_250262399.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M9412_RS08095 (M9412_08095) - 1697767..1698723 (+) 957 WP_250262400.1 RodZ family helix-turn-helix domain-containing protein -
  M9412_RS08100 (M9412_08100) ispG 1698750..1699853 (+) 1104 WP_250262401.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M9412_RS08105 (M9412_08105) hisS 1699883..1701154 (+) 1272 WP_250262402.1 histidine--tRNA ligase -
  M9412_RS08110 (M9412_08110) - 1701173..1701784 (+) 612 WP_203331614.1 YfgM family protein -
  M9412_RS08115 (M9412_08115) - 1701993..1702430 (+) 438 WP_010907449.1 isoprenylcysteine carboxylmethyltransferase family protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20921.47 Da        Isoelectric Point: 8.6020

>NTDB_id=692472 M9412_RS08090 WP_250262399.1 1697099..1697644(+) (pilF/pilF2) [Pasteurella multocida strain 35564]
MNRTFRFGIASIFSLFLSACVSQEGNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFYPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=692472 M9412_RS08090 WP_250262399.1 1697099..1697644(+) (pilF/pilF2) [Pasteurella multocida strain 35564]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTAACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCTCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGTGATGTATTAAA
TAATTATGGTACTTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGCCAAAGATGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTTATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.096

92.265

0.481
  pilF Glaesserella parasuis strain SC1401

44.865

100

0.459