Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M9421_RS08095 Genome accession   NZ_CP097788
Coordinates   1703040..1703585 (+) Length   181 a.a.
NCBI ID   WP_250262399.1    Uniprot ID   -
Organism   Pasteurella multocida strain 21317     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1698040..1708585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9421_RS08070 (M9421_08070) glnB 1698316..1698654 (+) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  M9421_RS08075 (M9421_08075) - 1698655..1699908 (+) 1254 WP_250262396.1 AI-2E family transporter -
  M9421_RS08080 (M9421_08080) - 1700026..1700268 (+) 243 WP_250262397.1 hypothetical protein -
  M9421_RS08085 (M9421_08085) - 1700241..1701635 (+) 1395 WP_250262398.1 tetratricopeptide repeat protein -
  M9421_RS08090 (M9421_08090) - 1701816..1703000 (+) 1185 WP_005752447.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M9421_RS08095 (M9421_08095) pilF/pilF2 1703040..1703585 (+) 546 WP_250262399.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M9421_RS08100 (M9421_08100) - 1703708..1704664 (+) 957 WP_250262400.1 RodZ family helix-turn-helix domain-containing protein -
  M9421_RS08105 (M9421_08105) ispG 1704691..1705794 (+) 1104 WP_250262401.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M9421_RS08110 (M9421_08110) hisS 1705824..1707095 (+) 1272 WP_250262402.1 histidine--tRNA ligase -
  M9421_RS08115 (M9421_08115) - 1707114..1707725 (+) 612 WP_203331614.1 YfgM family protein -
  M9421_RS08120 (M9421_08120) - 1707934..1708371 (+) 438 WP_010907449.1 isoprenylcysteine carboxylmethyltransferase family protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20921.47 Da        Isoelectric Point: 8.6020

>NTDB_id=692449 M9421_RS08095 WP_250262399.1 1703040..1703585(+) (pilF/pilF2) [Pasteurella multocida strain 21317]
MNRTFRFGIASIFSLFLSACVSQEGNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFYPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=692449 M9421_RS08095 WP_250262399.1 1703040..1703585(+) (pilF/pilF2) [Pasteurella multocida strain 21317]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTAACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCTCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGTGATGTATTAAA
TAATTATGGTACTTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGCCAAAGATGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTTATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.096

92.265

0.481
  pilF Glaesserella parasuis strain SC1401

44.865

100

0.459