Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M9411_RS02450 Genome accession   NZ_CP097787
Coordinates   537298..537843 (-) Length   181 a.a.
NCBI ID   WP_046333479.1    Uniprot ID   -
Organism   Pasteurella multocida strain 38725     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 532298..542843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9411_RS02425 (M9411_02425) - 532509..532946 (-) 438 WP_169029470.1 isoprenylcysteine carboxylmethyltransferase family protein -
  M9411_RS02430 (M9411_02430) - 533158..533769 (-) 612 WP_005725137.1 YfgM family protein -
  M9411_RS02435 (M9411_02435) hisS 533788..535059 (-) 1272 WP_250252273.1 histidine--tRNA ligase -
  M9411_RS02440 (M9411_02440) ispG 535089..536192 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M9411_RS02445 (M9411_02445) - 536219..537175 (-) 957 WP_250252275.1 RodZ family helix-turn-helix domain-containing protein -
  M9411_RS02450 (M9411_02450) pilF/pilF2 537298..537843 (-) 546 WP_046333479.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M9411_RS02455 (M9411_02455) - 537883..539067 (-) 1185 WP_010907446.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M9411_RS02460 (M9411_02460) - 539247..541397 (-) 2151 WP_250252277.1 tetratricopeptide repeat protein -
  M9411_RS02465 (M9411_02465) - 541515..542768 (-) 1254 WP_250252279.1 AI-2E family transporter -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20829.28 Da        Isoelectric Point: 7.8917

>NTDB_id=692410 M9411_RS02450 WP_046333479.1 537298..537843(-) (pilF/pilF2) [Pasteurella multocida strain 38725]
MNRTFRFGIASIFSLFLSACVSQEGNNTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGAFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=692410 M9411_RS02450 WP_046333479.1 537298..537843(-) (pilF/pilF2) [Pasteurella multocida strain 38725]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTAACAA
TACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTGCCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGATGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.096

92.265

0.481
  pilF Glaesserella parasuis strain SC1401

44.865

100

0.459