Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MF621_RS00810 Genome accession   NZ_CP097779
Coordinates   160763..161200 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain R 4.6     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 155763..166200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MF621_RS00770 (MF621_000154) - 156342..156467 (-) 126 Protein_137 hypothetical protein -
  MF621_RS00775 (MF621_000155) - 156620..157822 (+) 1203 WP_032857854.1 GTP-binding protein -
  MF621_RS00780 (MF621_000156) - 157887..159023 (+) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  MF621_RS00785 (MF621_000157) - 159038..159472 (+) 435 WP_003156592.1 RDD family protein -
  MF621_RS00790 (MF621_000158) - 159590..159865 (+) 276 WP_369719109.1 YckD family protein -
  MF621_RS00795 (MF621_000159) - 159969..160085 (+) 117 WP_015388718.1 family 1 glycosylhydrolase -
  MF621_RS00800 (MF621_000160) - 160084..160302 (+) 219 Protein_143 family 1 glycosylhydrolase -
  MF621_RS00805 (MF621_000161) nin/comJ 160343..160741 (-) 399 WP_014304317.1 competence protein ComJ Regulator
  MF621_RS00810 (MF621_000162) nucA/comI 160763..161200 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MF621_RS00815 (MF621_000163) hxlB 161559..162116 (-) 558 WP_003156587.1 6-phospho-3-hexuloisomerase -
  MF621_RS00820 (MF621_000164) hxlA 162113..162748 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  MF621_RS00825 (MF621_000165) - 162980..163342 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=692255 MF621_RS00810 WP_003156588.1 160763..161200(-) (nucA/comI) [Bacillus velezensis strain R 4.6]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=692255 MF621_RS00810 WP_003156588.1 160763..161200(-) (nucA/comI) [Bacillus velezensis strain R 4.6]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641