Detailed information NTG0691443

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M8232_RS20420
• Genome accession: NZ_CP097646
• Coordinates: 4211016..4211816 (+)
• Length: 266 a.a.
• NCBI ID: WP_117124802.1
• Uniprot ID: -
• Organism: Klebsiella quasipneumoniae strain NE1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4206016..4216816

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M8232_RS20385 (M8232_20385)	gspH	4206169..4206684 (+)	516	WP_004204416.1	type II secretion system minor pseudopilin GspH	-
M8232_RS20390 (M8232_20390)	gspI	4206681..4207046 (+)	366	WP_004204415.1	type II secretion system minor pseudopilin GspI	-
M8232_RS20395 (M8232_20395)	gspJ	4207043..4207639 (+)	597	WP_156234741.1	type II secretion system minor pseudopilin GspJ	-
M8232_RS20400 (M8232_20400)	gspK	4207636..4208616 (+)	981	WP_156234740.1	type II secretion system minor pseudopilin GspK	-
M8232_RS20405 (M8232_20405)	gspL	4208609..4209805 (+)	1197	WP_156234739.1	type II secretion system protein GspL	-
M8232_RS20410 (M8232_20410)	-	4209798..4210283 (+)	486	WP_004204409.1	type II secretion system protein M	-
M8232_RS20415 (M8232_20415)	-	4210273..4211001 (+)	729	WP_064151076.1	type II secretion system protein N	-
M8232_RS20420 (M8232_20420)	pilD	4211016..4211816 (+)	801	WP_117124802.1	A24 family peptidase	Machinery gene
M8232_RS20425 (M8232_20425)	hrpB	4211864..4214293 (-)	2430	WP_064151074.1	ATP-dependent helicase HrpB	-
M8232_RS20430 (M8232_20430)	thpR	4214366..4214902 (+)	537	WP_049116602.1	RNA 2',3'-cyclic phosphodiesterase	-
M8232_RS20435 (M8232_20435)	sfsA	4214902..4215618 (+)	717	WP_004204404.1	DNA/RNA nuclease SfsA	-
M8232_RS20440 (M8232_20440)	dksA	4215781..4216236 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29368.95 Da
• Isoelectric Point: 8.8894

>NTDB_id=691443 M8232_RS20420 WP_117124802.1 4211016..4211816(+) (pilD) [Klebsiella quasipneumoniae strain NE1]
MTTLAALSLHFPLIWYGFLLLFGLALGSFYNVVIYRLPRMLAQTADDEPLTLSTPGSSCPQCRQPIAWRDNIPLVSFLWL
GRRARCCQARISWSYPLTELTTGLLFILAGALLAPGLALAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQALPQVLLFASASGLMWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGVFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=691443 M8232_RS20420 WP_117124802.1 4211016..4211816(+) (pilD) [Klebsiella quasipneumoniae strain NE1]
ATGACGACATTAGCCGCATTATCGCTGCATTTCCCTCTTATCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTACCGTCTGCCGCGCATGCTGGCCCAGACCGCGGACGACGAGCCGCTAACCCTCAGCA
CGCCGGGCTCATCCTGTCCGCAGTGTCGCCAGCCGATAGCCTGGCGGGACAATATCCCGCTAGTCAGCTTCCTCTGGCTA
GGCCGTCGCGCCCGCTGCTGTCAGGCGCGCATCTCGTGGAGCTATCCGCTGACCGAGCTGACCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCACCGGGACTGGCGCTGGCGGGCGGCCTGGTGCTGCTCTCCTTTCTGCTGATACTGGCGC
GGATTGATGCCCGGACCCAGCTGCTGCCAGACCGCCTGACCCTGCCCCTGCTGTGGGCCGGACTGCTGTTCAATCTTAAC
GAGGTGTACATTGCGCTGCCGGACGCGGTCGCCGGGGCGATGGCTGGTTATCTGGCGCTGTGGTCGGTCTATTGGCTGTT
CCGCCTGCTGACCGGCAAAGAGGCTCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGCTCTGCCGCAGGTGCTGTTGTTCGCCTCGGCCAGCGGGCTGATGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGAGGGGGCGTTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	45.818	100	0.474
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.678	98.12	0.429