Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M8856_RS06430 Genome accession   NZ_CP097622
Coordinates   1397186..1397731 (-) Length   181 a.a.
NCBI ID   WP_005758192.1    Uniprot ID   -
Organism   Pasteurella multocida strain P2723     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1392186..1402731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8856_RS06405 (M8856_06405) - 1392397..1392833 (-) 437 Protein_1230 isoprenylcysteine carboxylmethyltransferase family protein -
  M8856_RS06410 (M8856_06410) - 1393046..1393657 (-) 612 WP_071522791.1 YfgM family protein -
  M8856_RS06415 (M8856_06415) hisS 1393676..1394947 (-) 1272 WP_010907448.1 histidine--tRNA ligase -
  M8856_RS06420 (M8856_06420) ispG 1394977..1396080 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M8856_RS06425 (M8856_06425) - 1396107..1397063 (-) 957 WP_005725132.1 RodZ family helix-turn-helix domain-containing protein -
  M8856_RS06430 (M8856_06430) pilF/pilF2 1397186..1397731 (-) 546 WP_005758192.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M8856_RS06435 (M8856_06435) - 1397770..1398953 (-) 1184 Protein_1236 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M8856_RS06440 (M8856_06440) - 1399132..1400388 (-) 1257 WP_250190372.1 AI-2E family transporter -
  M8856_RS06445 (M8856_06445) glnB 1400389..1400727 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  M8856_RS06450 (M8856_06450) mog 1400772..1401350 (-) 579 WP_010907443.1 molybdopterin adenylyltransferase -
  M8856_RS06455 (M8856_06455) - 1401376..1402305 (-) 930 WP_005725108.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20858.43 Da        Isoelectric Point: 8.1524

>NTDB_id=691321 M8856_RS06430 WP_005758192.1 1397186..1397731(-) (pilF/pilF2) [Pasteurella multocida strain P2723]
MNRTFRFGIASIFSLLLCACVSQESNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPSRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=691321 M8856_RS06430 WP_005758192.1 1397186..1397731(-) (pilF/pilF2) [Pasteurella multocida strain P2723]
ATGAACCGTACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTGCTTTGTGCTTGTGTGAGTCAAGAAAGTAACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCTGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGCCAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAGTAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.695

92.265

0.486
  pilF Glaesserella parasuis strain SC1401

45.946

100

0.47