Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M8854_RS06690 Genome accession   NZ_CP097618
Coordinates   1440712..1441257 (-) Length   181 a.a.
NCBI ID   WP_059245747.1    Uniprot ID   -
Organism   Pasteurella multocida strain P2095     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1435712..1446257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8854_RS06665 (M8854_06665) - 1435923..1436360 (-) 438 WP_005725139.1 isoprenylcysteine carboxylmethyltransferase family protein -
  M8854_RS06670 (M8854_06670) - 1436572..1437183 (-) 612 WP_005725137.1 YfgM family protein -
  M8854_RS06675 (M8854_06675) hisS 1437202..1438473 (-) 1272 WP_010907448.1 histidine--tRNA ligase -
  M8854_RS06680 (M8854_06680) ispG 1438503..1439606 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M8854_RS06685 (M8854_06685) - 1439633..1440589 (-) 957 WP_059245748.1 RodZ family helix-turn-helix domain-containing protein -
  M8854_RS06690 (M8854_06690) pilF/pilF2 1440712..1441257 (-) 546 WP_059245747.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M8854_RS06695 (M8854_06695) - 1441297..1442481 (-) 1185 WP_064964968.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M8854_RS06700 (M8854_06700) - 1442662..1444932 (-) 2271 WP_059245745.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20855.42 Da        Isoelectric Point: 8.1524

>NTDB_id=691279 M8854_RS06690 WP_059245747.1 1440712..1441257(-) (pilF/pilF2) [Pasteurella multocida strain P2095]
MNRTFRFGIASIFSLLLCACVSQESNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGAFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=691279 M8854_RS06690 WP_059245747.1 1440712..1441257(-) (pilF/pilF2) [Pasteurella multocida strain P2095]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTGCTTTGTGCTTGTGTGAGTCAAGAAAGTAACAA
AACGTTTGATTCACATGAGGCTGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCTGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTGCCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGATGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.096

92.265

0.481
  pilF Glaesserella parasuis strain SC1401

44.865

100

0.459