Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M8853_RS06480 Genome accession   NZ_CP097616
Coordinates   1418243..1418788 (-) Length   181 a.a.
NCBI ID   WP_064972673.1    Uniprot ID   -
Organism   Pasteurella multocida strain P1702     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1413243..1423788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8853_RS06455 (M8853_06455) - 1413454..1413891 (-) 438 WP_010907449.1 isoprenylcysteine carboxylmethyltransferase family protein -
  M8853_RS06460 (M8853_06460) - 1414103..1414714 (-) 612 WP_005725137.1 YfgM family protein -
  M8853_RS06465 (M8853_06465) hisS 1414733..1416004 (-) 1272 WP_064972674.1 histidine--tRNA ligase -
  M8853_RS06470 (M8853_06470) ispG 1416034..1417137 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M8853_RS06475 (M8853_06475) - 1417164..1418120 (-) 957 WP_059245748.1 RodZ family helix-turn-helix domain-containing protein -
  M8853_RS06480 (M8853_06480) pilF/pilF2 1418243..1418788 (-) 546 WP_064972673.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M8853_RS06485 (M8853_06485) - 1418828..1420012 (-) 1185 WP_010907446.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M8853_RS06490 (M8853_06490) - 1420191..1421444 (-) 1254 WP_005755688.1 AI-2E family transporter -
  M8853_RS06495 (M8853_06495) glnB 1421445..1421783 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  M8853_RS06500 (M8853_06500) mog 1421828..1422406 (-) 579 WP_005737641.1 molybdopterin adenylyltransferase -
  M8853_RS06505 (M8853_06505) - 1422432..1423361 (-) 930 WP_005725108.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20941.54 Da        Isoelectric Point: 7.8913

>NTDB_id=691259 M8853_RS06480 WP_064972673.1 1418243..1418788(-) (pilF/pilF2) [Pasteurella multocida strain P1702]
MTRVFQVWIASIFSLFLSACVSQEGNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALFHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGAFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=691259 M8853_RS06480 WP_064972673.1 1418243..1418788(-) (pilF/pilF2) [Pasteurella multocida strain P1702]
ATGACGCGCGTTTTTCAAGTATGGATAGCAAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTAACAA
AACGTTTGATTCACATGAGGCAGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATTCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTGCCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGATGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

50

97.238

0.486
  pilF Glaesserella parasuis strain SC1401

43.85

100

0.453