Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M8851_RS07270 Genome accession   NZ_CP097612
Coordinates   1519661..1520206 (-) Length   181 a.a.
NCBI ID   WP_005755689.1    Uniprot ID   -
Organism   Pasteurella multocida strain 33011     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1514661..1525206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8851_RS07245 (M8851_07245) - 1514872..1515309 (-) 438 WP_005755695.1 isoprenylcysteine carboxylmethyltransferase family protein -
  M8851_RS07250 (M8851_07250) - 1515521..1516132 (-) 612 WP_250192342.1 YfgM family protein -
  M8851_RS07255 (M8851_07255) hisS 1516151..1517422 (-) 1272 WP_005755693.1 histidine--tRNA ligase -
  M8851_RS07260 (M8851_07260) ispG 1517452..1518555 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M8851_RS07265 (M8851_07265) - 1518582..1519538 (-) 957 WP_005755691.1 RodZ domain-containing protein -
  M8851_RS07270 (M8851_07270) pilF/pilF2 1519661..1520206 (-) 546 WP_005755689.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M8851_RS07275 (M8851_07275) - 1520245..1521429 (-) 1185 WP_005752447.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M8851_RS07280 (M8851_07280) - 1521610..1522863 (-) 1254 WP_005755688.1 AI-2E family transporter -
  M8851_RS07285 (M8851_07285) glnB 1522864..1523202 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  M8851_RS07290 (M8851_07290) mog 1523247..1523825 (-) 579 WP_005755686.1 molybdopterin adenylyltransferase -
  M8851_RS07295 (M8851_07295) - 1523851..1524780 (-) 930 WP_005725108.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20875.37 Da        Isoelectric Point: 7.8258

>NTDB_id=691217 M8851_RS07270 WP_005755689.1 1519661..1520206(-) (pilF/pilF2) [Pasteurella multocida strain 33011]
MNRTFRFGIASIFSLFLCACVSQEGNNTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=691217 M8851_RS07270 WP_005755689.1 1519661..1520206(-) (pilF/pilF2) [Pasteurella multocida strain 33011]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTTCTTTGTGCTTGTGTGAGTCAAGAAGGTAACAA
TACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACTTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.096

92.265

0.481
  pilF Glaesserella parasuis strain SC1401

45.405

100

0.464