Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M8850_RS07165 Genome accession   NZ_CP097610
Coordinates   1504700..1505245 (-) Length   181 a.a.
NCBI ID   WP_005755689.1    Uniprot ID   -
Organism   Pasteurella multocida strain 32985     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1499700..1510245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8850_RS07140 (M8850_07140) - 1499911..1500348 (-) 438 WP_005755695.1 isoprenylcysteine carboxylmethyltransferase family protein -
  M8850_RS07145 (M8850_07145) - 1500560..1501171 (-) 612 WP_005725137.1 YfgM family protein -
  M8850_RS07150 (M8850_07150) hisS 1501190..1502461 (-) 1272 WP_005755693.1 histidine--tRNA ligase -
  M8850_RS07155 (M8850_07155) ispG 1502491..1503594 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M8850_RS07160 (M8850_07160) - 1503621..1504577 (-) 957 WP_005755691.1 RodZ domain-containing protein -
  M8850_RS07165 (M8850_07165) pilF/pilF2 1504700..1505245 (-) 546 WP_005755689.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M8850_RS07170 (M8850_07170) - 1505284..1506468 (-) 1185 WP_250190557.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M8850_RS07175 (M8850_07175) - 1506649..1507902 (-) 1254 WP_005755688.1 AI-2E family transporter -
  M8850_RS07180 (M8850_07180) glnB 1507903..1508241 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  M8850_RS07185 (M8850_07185) mog 1508286..1508864 (-) 579 WP_005755686.1 molybdopterin adenylyltransferase -
  M8850_RS07190 (M8850_07190) - 1508890..1509819 (-) 930 WP_005725108.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20875.37 Da        Isoelectric Point: 7.8258

>NTDB_id=691195 M8850_RS07165 WP_005755689.1 1504700..1505245(-) (pilF/pilF2) [Pasteurella multocida strain 32985]
MNRTFRFGIASIFSLFLCACVSQEGNNTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=691195 M8850_RS07165 WP_005755689.1 1504700..1505245(-) (pilF/pilF2) [Pasteurella multocida strain 32985]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTTCTTTGTGCTTGTGTGAGTCAAGAAGGTAACAA
TACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACTTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.096

92.265

0.481
  pilF Glaesserella parasuis strain SC1401

45.405

100

0.464