Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M8848_RS06245 Genome accession   NZ_CP097608
Coordinates   1369425..1369970 (-) Length   181 a.a.
NCBI ID   WP_250193524.1    Uniprot ID   -
Organism   Pasteurella multocida strain 11205     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1364425..1374970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8848_RS06220 (M8848_06220) - 1364635..1365072 (-) 438 WP_169029470.1 isoprenylcysteine carboxylmethyltransferase family protein -
  M8848_RS06225 (M8848_06225) - 1365285..1365896 (-) 612 WP_005725137.1 YfgM family protein -
  M8848_RS06230 (M8848_06230) hisS 1365915..1367186 (-) 1272 WP_010907448.1 histidine--tRNA ligase -
  M8848_RS06235 (M8848_06235) ispG 1367216..1368319 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M8848_RS06240 (M8848_06240) - 1368346..1369302 (-) 957 WP_250193523.1 RodZ family helix-turn-helix domain-containing protein -
  M8848_RS06245 (M8848_06245) pilF/pilF2 1369425..1369970 (-) 546 WP_250193524.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M8848_RS06250 (M8848_06250) - 1370009..1371193 (-) 1185 WP_005752447.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M8848_RS06255 (M8848_06255) - 1371373..1373091 (-) 1719 WP_250193525.1 tetratricopeptide repeat protein -
  M8848_RS06260 (M8848_06260) - 1373209..1374462 (-) 1254 WP_005755688.1 AI-2E family transporter -
  M8848_RS06265 (M8848_06265) glnB 1374463..1374801 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20933.48 Da        Isoelectric Point: 8.2666

>NTDB_id=691149 M8848_RS06245 WP_250193524.1 1369425..1369970(-) (pilF/pilF2) [Pasteurella multocida strain 11205]
MNRTFRFGIASIFSLFLSACVSQEGNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALFHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=691149 M8848_RS06245 WP_250193524.1 1369425..1369970(-) (pilF/pilF2) [Pasteurella multocida strain 11205]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTAACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATTCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGATGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.695

92.265

0.486
  pilF Glaesserella parasuis strain SC1401

45.405

100

0.464