Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M8846_RS06280 Genome accession   NZ_CP097606
Coordinates   1389945..1390490 (-) Length   181 a.a.
NCBI ID   WP_169323504.1    Uniprot ID   -
Organism   Pasteurella multocida strain 10957     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1384945..1395490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8846_RS06255 (M8846_06255) - 1385156..1385593 (-) 438 WP_005755695.1 isoprenylcysteine carboxylmethyltransferase family protein -
  M8846_RS06260 (M8846_06260) - 1385805..1386416 (-) 612 WP_005725137.1 YfgM family protein -
  M8846_RS06265 (M8846_06265) hisS 1386435..1387706 (-) 1272 WP_170385499.1 histidine--tRNA ligase -
  M8846_RS06270 (M8846_06270) ispG 1387736..1388839 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M8846_RS06275 (M8846_06275) - 1388866..1389822 (-) 957 WP_014326355.1 RodZ family helix-turn-helix domain-containing protein -
  M8846_RS06280 (M8846_06280) pilF/pilF2 1389945..1390490 (-) 546 WP_169323504.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M8846_RS06285 (M8846_06285) - 1390530..1391714 (-) 1185 WP_250191496.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M8846_RS06290 (M8846_06290) - 1391918..1394992 (-) 3075 WP_250191497.1 HsdR family type I site-specific deoxyribonuclease -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20972.52 Da        Isoelectric Point: 8.6120

>NTDB_id=691105 M8846_RS06280 WP_169323504.1 1389945..1390490(-) (pilF/pilF2) [Pasteurella multocida strain 10957]
MNRTFRFRIASIFSLFLSACVSQEGNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=691105 M8846_RS06280 WP_169323504.1 1389945..1390490(-) (pilF/pilF2) [Pasteurella multocida strain 10957]
ATGAACCGCACTTTTCGATTTAGGATCGCGAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTAACAA
AACGTTTGATTCACATGAGGCAGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.695

92.265

0.486
  pilF Glaesserella parasuis strain SC1401

45.405

100

0.464