Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M8993_RS06190 Genome accession   NZ_CP097605
Coordinates   1373672..1374217 (-) Length   181 a.a.
NCBI ID   WP_169029445.1    Uniprot ID   -
Organism   Pasteurella multocida strain 10185     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1368672..1379217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8993_RS06165 (M8993_06165) - 1368883..1369320 (-) 438 WP_169029470.1 isoprenylcysteine carboxylmethyltransferase family protein -
  M8993_RS06170 (M8993_06170) - 1369532..1370143 (-) 612 WP_005725137.1 YfgM family protein -
  M8993_RS06175 (M8993_06175) hisS 1370162..1371433 (-) 1272 WP_005758195.1 histidine--tRNA ligase -
  M8993_RS06180 (M8993_06180) ispG 1371463..1372566 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M8993_RS06185 (M8993_06185) - 1372593..1373549 (-) 957 WP_005725132.1 RodZ family helix-turn-helix domain-containing protein -
  M8993_RS06190 (M8993_06190) pilF/pilF2 1373672..1374217 (-) 546 WP_169029445.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M8993_RS06195 (M8993_06195) - 1374257..1375441 (-) 1185 WP_005752447.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M8993_RS06200 (M8993_06200) - 1375622..1376875 (-) 1254 WP_005755688.1 AI-2E family transporter -
  M8993_RS06205 (M8993_06205) glnB 1376876..1377214 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  M8993_RS06210 (M8993_06210) mog 1377259..1377837 (-) 579 WP_010907443.1 molybdopterin adenylyltransferase -
  M8993_RS06215 (M8993_06215) - 1377863..1378792 (-) 930 WP_005725108.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20909.50 Da        Isoelectric Point: 7.8913

>NTDB_id=691083 M8993_RS06190 WP_169029445.1 1373672..1374217(-) (pilF/pilF2) [Pasteurella multocida strain 10185]
MTRVFQIWIASIFSLFLAACVSQEGNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGAFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDTVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=691083 M8993_RS06190 WP_169029445.1 1373672..1374217(-) (pilF/pilF2) [Pasteurella multocida strain 10185]
ATGACGCGCGTTTTTCAAATATGGATAGCAAGCATTTTTTCTCTTTTTCTTGCTGCCTGTGTGAGTCAAGAAGGTAACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCTCATGCCCCAAATTACTATTTATCTCATGCTGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTGCCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGCCAAAGACACTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

49.432

97.238

0.481
  pilF Glaesserella parasuis strain SC1401

43.85

100

0.453