Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   M8331_RS06505 Genome accession   NZ_CP097604
Coordinates   1399032..1399577 (-) Length   181 a.a.
NCBI ID   WP_083004641.1    Uniprot ID   -
Organism   Pasteurella multocida strain 10159     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1394032..1404577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8331_RS06485 (M8331_06485) - 1394895..1395506 (-) 612 WP_083004649.1 YfgM family protein -
  M8331_RS06490 (M8331_06490) hisS 1395525..1396796 (-) 1272 WP_083004646.1 histidine--tRNA ligase -
  M8331_RS06495 (M8331_06495) ispG 1396826..1397929 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M8331_RS06500 (M8331_06500) - 1397956..1398909 (-) 954 WP_083004644.1 RodZ family helix-turn-helix domain-containing protein -
  M8331_RS06505 (M8331_06505) pilF/pilF2 1399032..1399577 (-) 546 WP_083004641.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M8331_RS06510 (M8331_06510) - 1399617..1400801 (-) 1185 WP_083004640.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M8331_RS06515 (M8331_06515) - 1400980..1402233 (-) 1254 WP_005725114.1 AI-2E family transporter -
  M8331_RS06520 (M8331_06520) glnB 1402234..1402572 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  M8331_RS06525 (M8331_06525) mog 1402617..1403195 (-) 579 WP_250190750.1 molybdopterin adenylyltransferase -
  M8331_RS06530 (M8331_06530) - 1403221..1404150 (-) 930 WP_005725108.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20819.37 Da        Isoelectric Point: 7.9056

>NTDB_id=691062 M8331_RS06505 WP_083004641.1 1399032..1399577(-) (pilF/pilF2) [Pasteurella multocida strain 10159]
MTRVFQLGIASIFSLFLSACVSQEGHKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=691062 M8331_RS06505 WP_083004641.1 1399032..1399577(-) (pilF/pilF2) [Pasteurella multocida strain 10159]
ATGACGCGCGTTTTTCAATTAGGGATAGCAAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTCACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGTGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGCCAAAGATGCTGTCCGTTATCAACAA
AATTTGCAACACTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.695

92.265

0.486
  pilF Glaesserella parasuis strain SC1401

44.385

100

0.459