Detailed information    

insolico Bioinformatically predicted

Overview

Name   phrF   Type   Regulator
Locus tag   M8966_RS03265 Genome accession   NZ_CP097589
Coordinates   639860..639979 (+) Length   39 a.a.
NCBI ID   WP_007407639.1    Uniprot ID   A3KLD5
Organism   Bacillus velezensis strain UA0311     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context

Location: 634860..644979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8966_RS03245 - 635211..635722 (+) 512 Protein_646 bacteriocin biosynthesis protein AlbG -
  M8966_RS03250 - 635729..637105 (-) 1377 WP_012118684.1 YncE family protein -
  M8966_RS03255 - 637145..638500 (-) 1356 WP_012118685.1 YncE family protein -
  M8966_RS03260 rapF 638731..639876 (+) 1146 WP_012118686.1 tetratricopeptide repeat protein Regulator
  M8966_RS03265 phrF 639860..639979 (+) 120 WP_007407639.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  M8966_RS03270 - 640177..640380 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  M8966_RS03275 - 640394..640597 (+) 204 WP_007407641.1 hypothetical protein -
  M8966_RS03280 - 640632..640817 (+) 186 WP_007407642.1 DUF4177 domain-containing protein -
  M8966_RS03285 - 641003..641284 (+) 282 WP_007407643.1 hypothetical protein -
  M8966_RS03290 - 641328..641840 (+) 513 WP_023357177.1 stage II sporulation protein M -
  M8966_RS03295 - 642431..642649 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  M8966_RS03300 speB 642817..643689 (-) 873 WP_007614448.1 agmatinase -
  M8966_RS03305 speE 643748..644578 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 39 a.a.        Molecular weight: 4295.19 Da        Isoelectric Point: 10.0233

>NTDB_id=690612 M8966_RS03265 WP_007407639.1 639860..639979(+) (phrF) [Bacillus velezensis strain UA0311]
MKLKYKLALTCLAISTVFVSAEIASAPKHQFEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=690612 M8966_RS03265 WP_007407639.1 639860..639979(+) (phrF) [Bacillus velezensis strain UA0311]
ATGAAATTGAAGTATAAATTAGCACTTACTTGCTTGGCAATAAGCACTGTTTTTGTTTCAGCCGAAATCGCAAGTGCACC
GAAACATCAATTTGAAGTTGCTCAGCGCGGAATGATTTAA

Domains

Predicted by InterproScan.

Rap-phr extracellular signalling (PhrC_PhrF)

(3-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3KLD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

74.074

69.231

0.513
  phrC Bacillus subtilis subsp. subtilis str. 168

46.154

100

0.462