Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   M8961_RS16200 Genome accession   NZ_CP097588
Coordinates   3270528..3270965 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain UA0323     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3265528..3275965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8961_RS16185 - 3268386..3268748 (-) 363 WP_012116770.1 helix-turn-helix domain-containing protein -
  M8961_RS16190 hxlA 3268980..3269615 (+) 636 WP_012116769.1 3-hexulose-6-phosphate synthase -
  M8961_RS16195 hxlB 3269612..3270169 (+) 558 WP_007409354.1 6-phospho-3-hexuloisomerase -
  M8961_RS16200 nucA/comI 3270528..3270965 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  M8961_RS16205 nin/comJ 3270986..3271384 (+) 399 WP_007609299.1 competence protein ComJ Regulator
  M8961_RS16210 - 3271425..3272861 (-) 1437 WP_012116768.1 family 1 glycosylhydrolase -
  M8961_RS16215 - 3272965..3273288 (-) 324 WP_007409357.1 YckD family protein -
  M8961_RS16220 - 3273361..3273795 (-) 435 WP_012116767.1 RDD family protein -
  M8961_RS16225 - 3273810..3274946 (-) 1137 WP_012116766.1 zinc-dependent alcohol dehydrogenase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=690593 M8961_RS16200 WP_003156588.1 3270528..3270965(+) (nucA/comI) [Bacillus velezensis strain UA0323]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=690593 M8961_RS16200 WP_003156588.1 3270528..3270965(+) (nucA/comI) [Bacillus velezensis strain UA0323]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641