Detailed information    

insolico Bioinformatically predicted

Overview

Name   phrF   Type   Regulator
Locus tag   M8957_RS10815 Genome accession   NZ_CP097587
Coordinates   2271723..2271842 (+) Length   39 a.a.
NCBI ID   WP_007407639.1    Uniprot ID   A3KLD5
Organism   Bacillus velezensis strain UA0276     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context

Location: 2266723..2276842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8957_RS10795 - 2267074..2267585 (+) 512 Protein_2147 bacteriocin biosynthesis protein AlbG -
  M8957_RS10800 - 2267592..2268968 (-) 1377 WP_012118684.1 YncE family protein -
  M8957_RS10805 - 2269008..2270363 (-) 1356 WP_012118685.1 YncE family protein -
  M8957_RS10810 rapF 2270594..2271739 (+) 1146 WP_012118686.1 tetratricopeptide repeat protein Regulator
  M8957_RS10815 phrF 2271723..2271842 (+) 120 WP_007407639.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  M8957_RS10820 - 2272040..2272243 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  M8957_RS10825 - 2272257..2272460 (+) 204 WP_007407641.1 hypothetical protein -
  M8957_RS10830 - 2272495..2272680 (+) 186 WP_007407642.1 DUF4177 domain-containing protein -
  M8957_RS10835 - 2272866..2273147 (+) 282 WP_007407643.1 hypothetical protein -
  M8957_RS10840 - 2273191..2273703 (+) 513 WP_023357177.1 stage II sporulation protein M -
  M8957_RS10845 - 2274294..2274512 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  M8957_RS10850 speB 2274680..2275552 (-) 873 WP_007614448.1 agmatinase -
  M8957_RS10855 speE 2275611..2276441 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 39 a.a.        Molecular weight: 4295.19 Da        Isoelectric Point: 10.0233

>NTDB_id=690491 M8957_RS10815 WP_007407639.1 2271723..2271842(+) (phrF) [Bacillus velezensis strain UA0276]
MKLKYKLALTCLAISTVFVSAEIASAPKHQFEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=690491 M8957_RS10815 WP_007407639.1 2271723..2271842(+) (phrF) [Bacillus velezensis strain UA0276]
ATGAAATTGAAGTATAAATTAGCACTTACTTGCTTGGCAATAAGCACTGTTTTTGTTTCAGCCGAAATCGCAAGTGCACC
GAAACATCAATTTGAAGTTGCTCAGCGCGGAATGATTTAA

Domains

Predicted by InterproScan.

Rap-phr extracellular signalling (PhrC_PhrF)

(3-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3KLD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

74.074

69.231

0.513
  phrC Bacillus subtilis subsp. subtilis str. 168

46.154

100

0.462