Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   ORP33_RS08455 Genome accession   NZ_CP113256
Coordinates   1619155..1621101 (+) Length   648 a.a.
NCBI ID   WP_015252109.1    Uniprot ID   -
Organism   Bacillus subtilis strain K1     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 1614155..1626101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ORP33_RS08430 (ORP33_08305) def 1614525..1615007 (+) 483 WP_029726494.1 peptide deformylase -
  ORP33_RS08435 (ORP33_08310) fmt 1615012..1615965 (+) 954 WP_029726493.1 methionyl-tRNA formyltransferase -
  ORP33_RS08440 (ORP33_08315) rsmB 1615952..1617295 (+) 1344 WP_225721989.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  ORP33_RS08445 (ORP33_08320) rlmN 1617299..1618390 (+) 1092 WP_015252110.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ORP33_RS08450 (ORP33_08325) prpC 1618397..1619161 (+) 765 WP_014479762.1 protein-serine/threonine phosphatase PrpC -
  ORP33_RS08455 (ORP33_08330) stkP 1619155..1621101 (+) 1947 WP_015252109.1 serine/threonine protein kinase PrkC Regulator
  ORP33_RS08460 (ORP33_08335) rsgA 1621116..1622012 (+) 897 WP_015715871.1 ribosome small subunit-dependent GTPase A -
  ORP33_RS08465 (ORP33_08340) rpe 1622017..1622670 (+) 654 WP_029726492.1 ribulose-phosphate 3-epimerase -
  ORP33_RS08470 (ORP33_08345) thiN 1622743..1623387 (+) 645 WP_014479765.1 thiamine diphosphokinase -
  ORP33_RS08475 (ORP33_08350) spoVM 1623459..1623539 (+) 81 WP_003221545.1 stage V sporulation protein SpoVM -
  ORP33_RS08480 (ORP33_08355) rpmB 1623612..1623800 (-) 189 WP_003221548.1 50S ribosomal protein L28 -
  ORP33_RS08485 (ORP33_08360) yloU 1624077..1624439 (+) 363 WP_003232054.1 Asp23/Gls24 family envelope stress response protein -

Sequence

Protein


Download         Length: 648 a.a.        Molecular weight: 71880.56 Da        Isoelectric Point: 4.7871

>NTDB_id=690393 ORP33_RS08455 WP_015252109.1 1619155..1621101(+) (stkP) [Bacillus subtilis strain K1]
MLIGKRISGRYQILRVIGGGGMANVYLAEDIILDREVAIKILRFDYANDNEFIRRFRREAQSASSLDHPNIVSIYDLGEE
DDIYYIVMEYVEGMTLKEYITANGPLHPKEALNIMEQIVSAIAHAHQNQIVHRDIKPHNILIDHMGNIKVTDFGIATALS
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGRIPFDGESAVSIALKHLQAETPSAKRWNPSVPQS
VENIILKATAKDPFHRYETAEDMEADIKTAFDADRLNEKRFTIQEDEEMTKAIPIIKDEELAKAAGEKEAEVTTAQENKT
KKNGKRKKWPWVLLTICLVFITAGILAVTVFPSLFMPKDVKIPDVSGMEYEKAAGLLEKEGLQVDSEVLEISDEKIEEGL
MVKTDPKADTTVKEGATVTLYKSTGKAKTEIGDVTGQTVDQAKKALKDQGFKHVTVNEVNDEKNAGTVIDQNPSAGTELV
PSEDQVKLTVSIGPEDITLRDLKTYSKEAASGYLEDNGLKLVEKEAYSDDVPEGQVVKQKPAAGTAVKPGNEVEVTFSLG
PEKKPAKTVKEKVKIPYEPENEGDELQVQIAVDDADHSISDTYEEFKIKEPTERTIELKIEPGQKGYYQVMVNNKVVSYK
TIEYPKDE

Nucleotide


Download         Length: 1947 bp        

>NTDB_id=690393 ORP33_RS08455 WP_015252109.1 1619155..1621101(+) (stkP) [Bacillus subtilis strain K1]
GTGCTAATCGGCAAGCGGATCAGCGGGCGTTACCAAATTCTCCGCGTTATAGGCGGCGGGGGAATGGCCAACGTGTATTT
AGCTGAGGATATCATTCTAGACCGTGAAGTCGCAATTAAAATCCTGCGGTTTGACTATGCAAATGACAATGAATTTATCA
GGCGTTTCCGCAGAGAAGCCCAATCCGCATCAAGCCTCGATCACCCGAATATTGTCAGCATTTATGATTTGGGCGAGGAA
GATGATATTTATTATATTGTCATGGAATACGTTGAAGGCATGACGCTTAAAGAATACATAACAGCAAATGGGCCGCTTCA
CCCTAAAGAAGCGCTGAACATCATGGAGCAAATTGTCTCAGCCATCGCTCATGCCCATCAAAACCAGATTGTTCACAGAG
ACATCAAGCCGCATAACATTTTGATTGATCATATGGGAAATATCAAAGTAACGGATTTTGGAATTGCGACGGCACTAAGT
TCGACCACAATCACCCATACCAATTCAGTTCTGGGCTCGGTCCATTACTTATCACCTGAACAGGCCCGGGGCGGCTTAGC
CACAAAAAAATCGGATATTTATGCGCTTGGAATCGTTCTATTTGAGCTTTTAACCGGCCGTATTCCGTTTGATGGAGAGT
CAGCAGTCAGCATCGCCTTGAAGCATCTTCAAGCGGAAACTCCTTCGGCAAAAAGGTGGAATCCATCGGTCCCCCAAAGC
GTTGAAAACATCATACTCAAGGCAACTGCCAAAGATCCTTTTCATCGCTACGAAACGGCTGAAGACATGGAAGCAGACAT
AAAAACAGCTTTTGATGCCGACAGACTCAATGAAAAGAGATTTACGATTCAAGAAGATGAAGAAATGACAAAAGCGATAC
CTATCATTAAAGATGAAGAACTTGCTAAAGCTGCTGGCGAAAAAGAAGCTGAAGTGACAACCGCACAAGAAAACAAAACA
AAGAAGAACGGCAAAAGAAAAAAGTGGCCGTGGGTTTTGCTCACGATATGCCTCGTTTTTATCACAGCTGGAATTCTTGC
TGTCACTGTTTTTCCGTCGCTTTTCATGCCTAAGGATGTCAAAATACCTGATGTCTCCGGAATGGAATACGAAAAAGCCG
CAGGGCTCTTGGAAAAAGAAGGTTTACAGGTTGATTCCGAGGTGTTGGAAATCTCAGATGAAAAAATTGAAGAGGGCCTG
ATGGTAAAAACGGATCCTAAAGCGGATACCACAGTCAAAGAAGGCGCCACGGTCACCCTTTATAAGAGCACCGGAAAAGC
AAAAACGGAGATCGGTGATGTGACAGGCCAAACGGTCGACCAGGCAAAAAAAGCGTTGAAGGACCAAGGGTTTAAACATG
TAACGGTAAATGAAGTGAATGACGAGAAAAACGCGGGCACTGTCATTGACCAAAATCCTTCAGCAGGGACTGAGCTTGTC
CCGAGTGAAGATCAAGTCAAACTTACAGTCAGTATCGGACCCGAAGACATTACGCTTAGAGACTTGAAAACCTACAGTAA
AGAAGCAGCGTCTGGATATCTGGAAGACAACGGATTGAAGCTTGTAGAAAAAGAAGCATACTCAGATGATGTTCCAGAAG
GACAGGTTGTCAAACAAAAACCAGCAGCAGGTACGGCAGTAAAGCCGGGAAACGAAGTTGAAGTGACATTCTCTCTCGGA
CCAGAGAAAAAACCTGCGAAAACAGTGAAAGAAAAGGTCAAGATCCCCTACGAACCAGAAAATGAAGGGGACGAGCTTCA
AGTGCAAATCGCGGTTGACGATGCGGATCACAGCATCTCTGACACTTACGAAGAATTTAAGATAAAAGAGCCGACTGAAC
GAACGATCGAACTAAAGATTGAACCCGGCCAAAAAGGGTACTATCAAGTAATGGTAAACAATAAAGTTGTCAGCTACAAA
ACCATTGAGTATCCAAAAGATGAATAA

Domains

Predicted by InterProScan.

Protein kinase domain (Pkinase)

(11-261)

PASTA domain (PASTA)

(360-423)

PASTA domain (PASTA)

(430-491)

PASTA domain (PASTA)

(498-558)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

40.517

89.506

0.363
  stkP Streptococcus pneumoniae D39

40.345

89.506

0.361
  stkP Streptococcus pneumoniae R6

40.345

89.506

0.361