Detailed information NTG0690375

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: M8964_RS00440
• Genome accession: NZ_CP097585
• Coordinates: 77800..78945 (-)
• Length: 381 a.a.
• NCBI ID: WP_012118686.1
• Uniprot ID: A3KLD4
• Organism: Bacillus velezensis strain UA1365
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 72800..83945

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M8964_RS00395	speE	73098..73928 (+)	831	WP_003151052.1	spermidine synthase	-
M8964_RS00400	speB	73987..74859 (+)	873	WP_007614448.1	agmatinase	-
M8964_RS00405	-	75027..75245 (+)	219	WP_003151056.1	LuxR C-terminal-related transcriptional regulator	-
M8964_RS00410	-	75836..76348 (-)	513	WP_023357177.1	stage II sporulation protein M	-
M8964_RS00415	-	76392..76673 (-)	282	WP_007407643.1	hypothetical protein	-
M8964_RS00420	-	76859..77044 (-)	186	WP_007407642.1	DUF4177 domain-containing protein	-
M8964_RS00425	-	77079..77282 (-)	204	WP_007407641.1	hypothetical protein	-
M8964_RS00430	-	77296..77499 (-)	204	WP_007407640.1	helix-turn-helix transcriptional regulator	-
M8964_RS00435	phrF	77697..77816 (-)	120	WP_007407639.1	PhrC/PhrF family phosphatase-inhibitory pheromone	Regulator
M8964_RS00440	rapF	77800..78945 (-)	1146	WP_012118686.1	tetratricopeptide repeat protein	Regulator
M8964_RS00445	-	79176..80531 (+)	1356	WP_012118685.1	YncE family protein	-
M8964_RS00450	-	80571..81947 (+)	1377	WP_012118684.1	YncE family protein	-
M8964_RS00455	-	81954..82465 (-)	512	Protein_90	bacteriocin biosynthesis protein AlbG	-
M8964_RS00460	-	82619..83047 (+)	429	WP_007407634.1	DUF1934 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45500.54 Da
• Isoelectric Point: 4.5407

>NTDB_id=690375 M8964_RS00440 WP_012118686.1 77800..78945(-) (rapF) [Bacillus velezensis strain UA1365]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQALLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIAEYEFLESLYVGEPDEEAI
MECFDFLRDKSMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=690375 M8964_RS00440 WP_012118686.1 77800..78945(-) (rapF) [Bacillus velezensis strain UA1365]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCTAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCTGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAACAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCACAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATCGCCGAGTATGAATTTTTAGAATCTTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATCGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

• Tetratricopeptide repeat (TPR_12) (189-253)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (5-65)

3D structure

• AlphaFold DB: A3KLD4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	78.215	100	0.782
rapC	Bacillus subtilis subsp. subtilis str. 168	56.579	99.738	0.564