Detailed information NTG0690124

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PAC42_RS28545
• Genome accession: NZ_CP097560
• Coordinates: 5952207..5953079 (+)
• Length: 290 a.a.
• NCBI ID: WP_003107301.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain C4.2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5947207..5958079

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PAC42_RS28530 (PAC42_28455)	-	5948590..5949054 (-)	465	WP_003122079.1	pilin	-
PAC42_RS28535 (PAC42_28460)	pilB	5949285..5950985 (+)	1701	WP_003122078.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PAC42_RS28540 (PAC42_28465)	pilC	5950989..5952206 (+)	1218	WP_003107299.1	type II secretion system F family protein	Machinery gene
PAC42_RS28545 (PAC42_28470)	pilD	5952207..5953079 (+)	873	WP_003107301.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
PAC42_RS28550 (PAC42_28475)	coaE	5953076..5953687 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
PAC42_RS28555 (PAC42_28480)	yacG	5953684..5953884 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
PAC42_RS28560 (PAC42_28485)	-	5953921..5954130 (-)	210	WP_003094660.1	hypothetical protein	-
PAC42_RS28565 (PAC42_28490)	tnpA	5954302..5954643 (+)	342	WP_023980254.1	IS66-like element accessory protein TnpA	-
PAC42_RS28570 (PAC42_28495)	tnpB	5954640..5954975 (+)	336	WP_023980255.1	IS66 family insertion sequence element accessory protein TnpB	-
PAC42_RS28575 (PAC42_28500)	tnpC	5955039..5956550 (+)	1512	WP_033983075.1	IS66 family transposase	-
PAC42_RS28580 (PAC42_28505)	-	5956611..5957300 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
PAC42_RS28585 (PAC42_28510)	-	5957297..5957767 (-)	471	WP_003103866.1	hypothetical protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31873.90 Da
• Isoelectric Point: 7.7068

>NTDB_id=690124 PAC42_RS28545 WP_003107301.1 5952207..5953079(+) (pilD) [Pseudomonas aeruginosa strain C4.2]
MTLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=690124 PAC42_RS28545 WP_003107301.1 5952207..5953079(+) (pilD) [Pseudomonas aeruginosa strain C4.2]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	48.921	95.862	0.469