Detailed information NTG0068979

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: SPYKS030_RS01385
• Genome accession: NZ_AP018337
• Coordinates: 253216..254286 (+)
• Length: 356 a.a.
• NCBI ID: WP_002992231.1
• Uniprot ID: A0A5S4TWS5
• Organism: Streptococcus pyogenes strain KS030
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 248216..259286

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPYKS030_RS01370 (SPYKS030_02220)	amiA	248744..250714 (+)	1971	WP_002992224.1	peptide ABC transporter substrate-binding protein	Regulator
SPYKS030_RS01375 (SPYKS030_02230)	amiC	250779..252281 (+)	1503	WP_011054186.1	ABC transporter permease	Regulator
SPYKS030_RS01380 (SPYKS030_02240)	amiD	252281..253207 (+)	927	WP_011054187.1	oligopeptide ABC transporter permease OppC	Regulator
SPYKS030_RS01385 (SPYKS030_02250)	amiE	253216..254286 (+)	1071	WP_002992231.1	ABC transporter ATP-binding protein	Regulator
SPYKS030_RS01390 (SPYKS030_02260)	amiF	254279..255202 (+)	924	WP_002986000.1	ABC transporter ATP-binding protein	Regulator
SPYKS030_RS10445	-	255240..255329 (-)	90	WP_109821088.1	IS3 family transposase	-
SPYKS030_RS10130 (SPYKS030_02270)	-	255350..255595 (-)	246	WP_030126956.1	hypothetical protein	-

Sequence

Protein

• Length: 356 a.a.
• Molecular weight: 39680.74 Da
• Isoelectric Point: 5.4774

>NTDB_id=68979 SPYKS030_RS01385 WP_002992231.1 253216..254286(+) (amiE) [Streptococcus pyogenes strain KS030]
MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFVGESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQEL
TDLKTNKEWAKIRGSKIATIFQDPMTSLSPIKTIGSQITEVIIKHQKVSNAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITHDLGVVASIADKVAVMYAGEIVE
FGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAIPGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAK
TWLLHPEAPKVQKPEVIQDLHQKILRKMSQQEEGNV

Nucleotide

• Length: 1071 bp

>NTDB_id=68979 SPYKS030_RS01385 WP_002992231.1 253216..254286(+) (amiE) [Streptococcus pyogenes strain KS030]
ATGACAAAAGAAAATAATGTAATCTTAACTGCCAAAGATGTGGTGGTAGAATTTGATGTGCGTGATCGTGTTTTAACAGC
TATTCGTAACGTCTCACTGGAACTTGTTGAAGGAGAAGTCCTTGCTTTTGTAGGGGAATCAGGCTCAGGTAAATCTGTTT
TAACAAAGACCTTTACAGGGATGTTGGAGTCTAATGGACGCATTGCTAATGGTTCAATTGTCTATCGTGGGCAAGAATTG
ACAGATTTAAAAACAAATAAAGAGTGGGCAAAGATTCGCGGCTCAAAAATCGCAACGATTTTCCAAGACCCAATGACCAG
TCTTAGTCCCATTAAAACTATCGGTAGCCAAATCACAGAAGTGATTATTAAGCACCAAAAAGTAAGTAATGCCAAAGCTA
AAGAAATGGCCCTTGATTACATGAATAAAGTGGGTATCCCAAATGCCAAAAAACGCTTTGAAGATTACCCATTTGAGTAT
TCAGGAGGAATGCGTCAACGTATTGTTATCGCTATTGCTTTAGCTTGTCGCCCAGATATTCTTATCTGTGATGAGCCAAC
AACAGCCCTTGATGTGACTATTCAAGCTCAAATCGTTGAGTTATTGAAGTCGCTTCAACGAGAATATCATTTCACCATTA
TCTTTATTACGCACGATTTAGGTGTTGTGGCAAGCATTGCAGATAAAGTGGCTGTCATGTATGCAGGAGAAATTGTTGAA
TTTGGAACAGTCGAAGAGATTTTCTATGATCCAAGACACCCCTATACATGGAGTTTGCTGTCTAGCTTACCGCAGTTGGC
AGATGAATCTGGTGAACTTTACGCTATTCCAGGAACGCCTCCATCACTTTATTCACCAATTATCGGAGATGCCTTTGCAC
TTCGCTCAGAATATGCTATGGTTTTAGACTTTGAAAAAGCACCTCCGGCGATTAACGTATCTGAGACTCATTGGGCCAAA
ACATGGCTTTTACACCCAGAGGCTCCAAAAGTTCAAAAACCAGAAGTCATTCAAGATTTGCATCAAAAAATCTTAAGGAA
AATGTCACAACAGGAGGAAGGAAATGTCTGA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (29-187)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (239-299)

3D structure

• AlphaFold DB: A0A5S4TWS5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	76.667	100	0.775
amiE	Streptococcus thermophilus LMG 18311	75.556	100	0.764
amiE	Streptococcus thermophilus LMD-9	75.556	100	0.764
oppD	Streptococcus mutans UA159	56.677	94.663	0.537