NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	M8X21_RS12860	Genome accession	NZ_CP097467
Coordinates	2626656..2627270 (-)	Length	204 a.a.
NCBI ID	WP_029973402.1	Uniprot ID	-
Organism	Bacillus velezensis strain HMB26553
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2621656..2632270

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M8X21_RS12830 (M8X21_12830)	rpsT	2621959..2622225 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
M8X21_RS12835 (M8X21_12835)	holA	2622188..2623283 (-)	1096	Protein_2484	DNA polymerase III subunit delta	-
M8X21_RS12840 (M8X21_12840)	-	2623323..2623474 (-)	152	Protein_2485	hypothetical protein	-
M8X21_RS12845 (M8X21_12845)	-	2623515..2623649 (+)	135	WP_003152870.1	YqzM family protein	-
M8X21_RS12850 (M8X21_12850)	comEC	2623668..2626019 (-)	2352	WP_029973403.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
M8X21_RS12855 (M8X21_12855)	-	2626020..2626589 (-)	570	WP_003152868.1	ComE operon protein 2	-
M8X21_RS12860 (M8X21_12860)	comEA	2626656..2627270 (-)	615	WP_029973402.1	helix-hairpin-helix domain-containing protein	Machinery gene
M8X21_RS12865 (M8X21_12865)	comER	2627329..2628150 (+)	822	WP_029973401.1	late competence protein ComER	-
M8X21_RS12870 (M8X21_12870)	-	2628219..2628956 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
M8X21_RS12875 (M8X21_12875)	rsfS	2628953..2629309 (-)	357	WP_007408260.1	ribosome silencing factor	-
M8X21_RS12880 (M8X21_12880)	yqeK	2629327..2629887 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
M8X21_RS12885 (M8X21_12885)	-	2629877..2630446 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
M8X21_RS12890 (M8X21_12890)	yhbY	2630457..2630747 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
M8X21_RS12895 (M8X21_12895)	aroE	2630741..2631583 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22240.29 Da Isoelectric Point: 5.0355

                                >NTDB_id=689643 M8X21_RS12860 WP_029973402.1 2626656..2627270(-) (comEA) [Bacillus velezensis strain HMB26553]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=689643 M8X21_RS12860 WP_029973402.1 2626656..2627270(-) (comEA) [Bacillus velezensis strain HMB26553]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT

GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0689643