Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   M8X21_RS12430 Genome accession   NZ_CP097467
Coordinates   2548854..2549924 (-) Length   356 a.a.
NCBI ID   WP_014418378.1    Uniprot ID   A0AAP4DGV9
Organism   Bacillus velezensis strain HMB26553     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2543854..2554924
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8X21_RS12380 (M8X21_12380) - 2543913..2544497 (-) 585 WP_012117977.1 signal peptidase I -
  M8X21_RS12385 (M8X21_12385) tapA 2544469..2545140 (-) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  M8X21_RS12390 (M8X21_12390) - 2545399..2545728 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  M8X21_RS12395 (M8X21_12395) - 2545769..2545948 (-) 180 WP_003153093.1 YqzE family protein -
  M8X21_RS12400 (M8X21_12400) comGG 2546005..2546382 (-) 378 WP_017418138.1 competence type IV pilus minor pilin ComGG Machinery gene
  M8X21_RS12405 (M8X21_12405) comGF 2546383..2546847 (-) 465 WP_233717055.1 competence type IV pilus minor pilin ComGF -
  M8X21_RS12410 (M8X21_12410) comGE 2546792..2547106 (-) 315 WP_029973875.1 competence type IV pilus minor pilin ComGE Machinery gene
  M8X21_RS12415 (M8X21_12415) comGD 2547090..2547527 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  M8X21_RS12420 (M8X21_12420) comGC 2547517..2547825 (-) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  M8X21_RS12425 (M8X21_12425) comGB 2547830..2548867 (-) 1038 WP_014418377.1 competence type IV pilus assembly protein ComGB Machinery gene
  M8X21_RS12430 (M8X21_12430) comGA 2548854..2549924 (-) 1071 WP_014418378.1 competence type IV pilus ATPase ComGA Machinery gene
  M8X21_RS12435 (M8X21_12435) - 2550117..2551067 (-) 951 WP_014418379.1 magnesium transporter CorA family protein -
  M8X21_RS12440 (M8X21_12440) - 2551213..2552514 (+) 1302 WP_029973873.1 hemolysin family protein -
  M8X21_RS12445 (M8X21_12445) - 2552552..2553382 (-) 831 WP_025649847.1 STAS domain-containing protein -
  M8X21_RS12455 (M8X21_12455) - 2553589..2553969 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  M8X21_RS12460 (M8X21_12460) - 2554206..2554451 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.81 Da        Isoelectric Point: 9.3485

>NTDB_id=689639 M8X21_RS12430 WP_014418378.1 2548854..2549924(-) (comGA) [Bacillus velezensis strain HMB26553]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=689639 M8X21_RS12430 WP_014418378.1 2548854..2549924(-) (comGA) [Bacillus velezensis strain HMB26553]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGTGCCGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCTCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374