Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   M6G44_RS06550 Genome accession   NZ_CP097377
Coordinates   1432263..1432811 (-) Length   182 a.a.
NCBI ID   WP_005617759.1    Uniprot ID   B3H245
Organism   Actinobacillus pleuropneumoniae strain GD2107     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 1431165..1432088 1432263..1432811 flank 175


Gene organization within MGE regions


Location: 1431165..1432811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M6G44_RS06545 (M6G44_06545) - 1431165..1432139 (-) 975 WP_012478345.1 IS30-like element ISApl1 family transposase -
  M6G44_RS06550 (M6G44_06550) pilF 1432263..1432811 (-) 549 WP_005617759.1 type IV pilus biogenesis/stability protein PilW Machinery gene

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20645.27 Da        Isoelectric Point: 6.9479

>NTDB_id=689320 M6G44_RS06550 WP_005617759.1 1432263..1432811(-) (pilF) [Actinobacillus pleuropneumoniae strain GD2107]
MNLAKFLRNLTACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQVHAEQAYQQALKSSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
AKVNEALSQLEKLDKSRAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=689320 M6G44_RS06550 WP_005617759.1 1432263..1432811(-) (pilF) [Actinobacillus pleuropneumoniae strain GD2107]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCCGTGTTGTTCGGTTGTTCTTCTCAGCTTCC
TACCTCTCAAACCACGTTTAATCGCTCGGAAGCGGTTAAAGCGCGTATCAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCATGATCAAAACGACTACTTACCTCACTCCGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGTTCATGCGGAACAGGCTTATCAGCAAGCATTGAAATCAAGTGATAATCGTCC
GGATGTCTTAAATAATTACGGAACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAACAACCTTATTATCATCAAGCCGATAGCCTCGAAAATATCGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGTAAACGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAATCAAGAGCGGCGGCACTACGCTAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B3H245

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

66.489

100

0.687
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.199

93.956

0.434