Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   M5E02_RS11425 Genome accession   NZ_CP097373
Coordinates   2238346..2239416 (-) Length   356 a.a.
NCBI ID   WP_252058306.1    Uniprot ID   -
Organism   Bacillus safensis strain AHB2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2233346..2244416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5E02_RS11375 (M5E02_11375) - 2233437..2234009 (-) 573 WP_252056286.1 signal peptidase I SipW -
  M5E02_RS11380 (M5E02_11380) tapA 2234062..2234589 (-) 528 WP_252056287.1 amyloid fiber anchoring/assembly protein TapA -
  M5E02_RS11385 (M5E02_11385) - 2234889..2235167 (+) 279 WP_252058305.1 DUF3889 domain-containing protein -
  M5E02_RS11390 (M5E02_11390) - 2235203..2235400 (-) 198 WP_095409000.1 YqzE family protein -
  M5E02_RS11395 (M5E02_11395) comGG 2235459..2235842 (-) 384 WP_252056288.1 competence type IV pilus minor pilin ComGG -
  M5E02_RS11400 (M5E02_11400) comGF 2235839..2236366 (-) 528 WP_286037361.1 competence type IV pilus minor pilin ComGF -
  M5E02_RS11405 (M5E02_11405) comGE 2236278..2236592 (-) 315 WP_252056290.1 competence type IV pilus minor pilin ComGE -
  M5E02_RS11410 (M5E02_11410) comGD 2236576..2237022 (-) 447 WP_252056291.1 competence type IV pilus minor pilin ComGD -
  M5E02_RS11415 (M5E02_11415) comGC 2237015..2237308 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  M5E02_RS11420 (M5E02_11420) comGB 2237325..2238365 (-) 1041 WP_252056292.1 competence type IV pilus assembly protein ComGB -
  M5E02_RS11425 (M5E02_11425) comGA 2238346..2239416 (-) 1071 WP_252058306.1 competence type IV pilus ATPase ComGA Machinery gene
  M5E02_RS11430 (M5E02_11430) - 2239594..2240469 (-) 876 WP_048239208.1 STAS domain-containing protein -
  M5E02_RS11440 (M5E02_11440) - 2240693..2241073 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  M5E02_RS11445 (M5E02_11445) - 2241285..2241527 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  M5E02_RS11450 (M5E02_11450) - 2241774..2242412 (-) 639 WP_252058307.1 MBL fold metallo-hydrolase -
  M5E02_RS11455 (M5E02_11455) - 2242569..2242742 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  M5E02_RS11460 (M5E02_11460) - 2242966..2243280 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40465.98 Da        Isoelectric Point: 9.5040

>NTDB_id=689207 M5E02_RS11425 WP_252058306.1 2238346..2239416(-) (comGA) [Bacillus safensis strain AHB2]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRKGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLKHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELICPFCGETCQLYCKLNRPFRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=689207 M5E02_RS11425 WP_252058306.1 2238346..2239416(-) (comGA) [Bacillus safensis strain AHB2]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCTTTCCGTGTTGACTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGAGGCTTATTGCACATTTTAAATTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGACCGCAAAATGGCTCACTAGCT
GTGATGTTAAGAAAAGGACAAGTGTTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGGTTATTATCATTTTTGA
AGCATTCGCATGGACTCATTTTATTTACTGGGCCAACAAATTCAGGGAAAACAACAACACTATATTCATTAATCCAGTTT
GCAAAAAAGAATTTTAATCGAAATATTATTACCCTTGAAGACCCTGTCGAAACGAGGAATGAAGAAGTGTTGCAGGTTCA
AGTGAATGAAAAAGCCGGCATCACGTATGCCGCTGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACGGCTAGAACGGCGATTAGGGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTTCAT
GCAAAAAATGCAAAAGGAGCCCTTTACCGCATGCTTGAATTTGGTGTCACAATGAATGAACTCGAACAAACCATGGTTGC
GATTGCTGCCCAGAGATTAATTGAGCTTATATGCCCTTTTTGCGGGGAAACATGTCAGCTTTACTGTAAATTAAATCGTC
CTTTCAGACGAACAAATGTATTTGAATTGCTCTATGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCCAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTACAAAGATTAATTCGGAAAGGAGTGGCACTCGGTTATTTATCAAAAAACACATATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

65.537

99.438

0.652