Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M5E02_RS00590 Genome accession   NZ_CP097373
Coordinates   109212..110591 (+) Length   459 a.a.
NCBI ID   WP_252057142.1    Uniprot ID   -
Organism   Bacillus safensis strain AHB2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 104212..115591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5E02_RS00570 (M5E02_00570) - 104554..105018 (+) 465 WP_003217184.1 CtsR family transcriptional regulator -
  M5E02_RS00575 (M5E02_00575) - 105033..105590 (+) 558 WP_070326871.1 UvrB/UvrC motif-containing protein -
  M5E02_RS00580 (M5E02_00580) - 105595..106686 (+) 1092 WP_252057141.1 protein arginine kinase -
  M5E02_RS00585 (M5E02_00585) clpC 106683..109118 (+) 2436 WP_048240170.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  M5E02_RS00590 (M5E02_00590) radA 109212..110591 (+) 1380 WP_252057142.1 DNA repair protein RadA Machinery gene
  M5E02_RS00595 (M5E02_00595) disA 110594..111673 (+) 1080 WP_024425581.1 DNA integrity scanning diadenylate cyclase DisA -
  M5E02_RS00600 (M5E02_00600) - 111827..112927 (+) 1101 WP_048240173.1 PIN/TRAM domain-containing protein -
  M5E02_RS00605 (M5E02_00605) ispD 112941..113630 (+) 690 WP_003217158.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  M5E02_RS00610 (M5E02_00610) ispF 113634..114110 (+) 477 WP_003216928.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49996.49 Da        Isoelectric Point: 7.3829

>NTDB_id=689176 M5E02_RS00590 WP_252057142.1 109212..110591(+) (radA) [Bacillus safensis strain AHB2]
MAKTKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPATRRSAFNHSVQTIQKPSPISAIETSEEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDKNQNVLYISGEESIKQTKLRADRLGIESASLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLTEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANIDGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=689176 M5E02_RS00590 WP_252057142.1 109212..110591(+) (radA) [Bacillus safensis strain AHB2]
ATGGCTAAGACAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGTCCAGGCTG
CGGCACGTGGAACAGTATGACAGAAGAGGTCGTTCGCAAAGAGCCGGCAACCCGTCGAAGTGCTTTTAATCATTCTGTTC
AAACCATTCAAAAACCTTCACCTATATCTGCAATTGAAACATCCGAAGAACCCCGAATTAAAACGAATTTAGAAGAATTT
AACCGAGTATTAGGAAGTGGAATTGTCAAAGGCTCTCTTGTTCTCATTGGCGGAGATCCTGGGATTGGAAAGTCCACATT
ATTATTACAAGTATCAGCACAACTCTCAGACAAAAATCAGAATGTGTTATACATATCTGGTGAGGAGTCCATTAAACAAA
CGAAGCTAAGAGCGGACCGCCTTGGCATTGAAAGCGCCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTGCTATACAGGAGATGAAACCTGCTTTTGTTGTGGTGGATTCGATTCAAACTGTTTATCAAAGCGATATTACGTCAGC
TCCCGGTTCTGTATCTCAAGTAAGAGAATGTACAGCACAGCTGATGAAAATTGCCAAGACAAATGGTATTCCTATTTTTA
TCGTTGGTCACGTCACCAAAGAAGGCTCGATCGCAGGTCCACGCCTTTTAGAGCATATGGTGGACACAGTTCTTTATTTT
GAAGGCGAGCGTCATCATACGTTTCGTATTTTACGGGCGGTCAAAAACCGATTTGGTTCAACGAATGAATTGGGAATTTT
TGAAATGAGAGAGGAAGGGCTCACAGAAGTATTAAACCCATCAGAAATCTTTTTAGAAGAGCGGTCGGCAGGTGTATCTG
GTTCGTGTGTTGTTGCCTCAATGGAAGGAACAAGACCCGTACTTGTTGAGATACAGGCGTTAATTTCCCCGACAAGCTTT
GGGAATCCGCGAAGAATGGCCACTGGCCTTGATCATAATCGAGTATCGCTCCTCATGGCAGTTTTAGAAAAACGCGTGGG
GCTGCTGCTGCAAAACCAAGATGCGTATTTAAAGGTCGCAGGCGGTGTGAAGCTTGACGAACCGGCGATTGACTTAGCTA
TTGCCGTCAGTATTGCATCAAGCTTTAAAGACGCAGCGCCGCATCAAGCGGATTGCTTTATAGGAGAGGTCGGTCTAACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAGCGAGTGCAGGAAGCTGCGAAACTAGGATTCAAGCGAATGTTTATTCC
TCAGGCGAATATAGATGGATGGAAAAAGCCGAGAGGGATTGAGTTAGTTGGTGTAGAAAATGTAGCGGAGGCACTTCGAA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

87.118

99.782

0.869
  radA Streptococcus mitis NCTC 12261

61.369

98.693

0.606
  radA Streptococcus pneumoniae Rx1

61.369

98.693

0.606
  radA Streptococcus pneumoniae D39

61.369

98.693

0.606
  radA Streptococcus pneumoniae R6

61.369

98.693

0.606
  radA Streptococcus pneumoniae TIGR4

61.369

98.693

0.606
  radA Streptococcus mitis SK321

61.369

98.693

0.606