Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   M4D78_RS12555 Genome accession   NZ_CP097329
Coordinates   2596501..2598513 (-) Length   670 a.a.
NCBI ID   WP_286390632.1    Uniprot ID   -
Organism   Pseudanabaena mucicola str. Chao 1806     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2591501..2603513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4D78_RS12540 miaA 2592905..2593804 (+) 900 WP_286390627.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  M4D78_RS12545 - 2593920..2595128 (-) 1209 WP_286390629.1 type II secretion system F family protein -
  M4D78_RS12550 - 2595191..2596345 (-) 1155 WP_286396836.1 type IV pilus twitching motility protein PilT -
  M4D78_RS12555 pilB/pilB1 2596501..2598513 (-) 2013 WP_286390632.1 GspE/PulE family protein Machinery gene
  M4D78_RS12560 - 2599105..2601147 (-) 2043 WP_286390635.1 D-alanyl-lipoteichoic acid biosynthesis protein DltD -
  M4D78_RS22190 - 2601408..2601917 (+) 510 Protein_2480 transposase -
  M4D78_RS12570 - 2602016..2603197 (+) 1182 WP_286390445.1 hypothetical protein -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74744.33 Da        Isoelectric Point: 5.2474

>NTDB_id=688604 M4D78_RS12555 WP_286390632.1 2596501..2598513(-) (pilB/pilB1) [Pseudanabaena mucicola str. Chao 1806]
MNTSDQAPTTAASRNRTKALAKRGGRTPFESKLIQAGHATSDQFDRAVKDSQEQNVPFLNALEQILGQQLAPDVTRYYKR
QQLFELRVLYGIEPIDPDVEVDKFDIPTLCALLDSNILPISSCRDCEMLPLVKTENSITLAVVAPDSYKVQNEIARLLKN
ITVVRRVIAREDFHRVFDNIVQFQVNKSAKAEGSISDEDLQVKDDVFGEDISQSDEGDELMVGGENSAPIISLVDKILVR
ALKEGCSDIHIEPQEKDLCIRLRKDGVLREYFFLSENKRLPKSIINAVISRFKIISNLNIAEKRVPQDGKLKRNFQGRRV
DFRVNTCPTQNGEKVCLRILDNSNTQLGLDKLISDPESLELMQSFAQRPYGLILVTGPTGSGKTTTLYSTLAECNDPGIN
ISTAEDPVEYNLPGLTQCQVLREKGMTFASILRAFLRQDPDVILVGETRDAETAKTAIEAALTGHLVLTTLHTNDAPSCI
ARLEEMGVEPFMASTAIIGVLAQRLLRRICDNCRIPYNPSQEELDRFGLPSSDLERTFYRANIVNRDAMLPGQRVCPKCN
GSGYKGRAGVYEIMKMTERLQSAINKGATTEVIKEIAVQEGMKTLMAYAFDLVRQGTTTLDEVLRVVYTDKGREAEERAR
RGKGLECDNCDAMLTPETVECPYCTTPRTF

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=688604 M4D78_RS12555 WP_286390632.1 2596501..2598513(-) (pilB/pilB1) [Pseudanabaena mucicola str. Chao 1806]
ATGAATACATCAGATCAAGCGCCAACCACAGCCGCCAGCAGAAATAGAACCAAAGCTCTTGCCAAACGAGGAGGTCGTAC
TCCCTTTGAGAGCAAACTCATCCAAGCAGGACATGCTACTTCCGACCAGTTCGATCGCGCCGTCAAGGATAGCCAAGAAC
AGAACGTTCCATTTCTCAATGCCCTAGAGCAAATTTTGGGGCAACAACTCGCCCCCGACGTTACCCGATACTATAAACGC
CAGCAACTTTTTGAACTACGGGTTCTTTACGGTATCGAGCCAATTGATCCCGATGTTGAAGTTGATAAATTTGATATCCC
TACCCTCTGTGCCTTACTAGATTCCAATATTTTACCCATCTCCAGTTGCCGCGACTGTGAGATGCTACCGCTAGTTAAAA
CTGAAAATAGCATTACCCTAGCTGTAGTTGCCCCAGACAGTTATAAAGTACAAAACGAGATTGCGCGGCTACTCAAAAAT
ATCACTGTGGTTAGGCGAGTGATTGCCCGCGAAGATTTCCATCGTGTATTTGACAACATCGTCCAGTTTCAAGTCAACAA
GAGTGCCAAGGCGGAAGGATCTATCAGTGACGAAGATCTCCAAGTTAAAGATGATGTCTTTGGCGAGGATATTTCGCAAT
CGGATGAGGGTGATGAACTCATGGTTGGTGGTGAAAACTCCGCCCCAATTATCAGTCTCGTTGATAAAATTCTTGTCAGA
GCACTCAAGGAAGGATGTTCCGATATCCACATTGAGCCACAGGAAAAAGACCTATGCATTCGCTTGCGGAAAGATGGCGT
TCTTCGTGAATACTTCTTCCTGAGCGAGAACAAGCGTTTGCCCAAGAGCATTATCAATGCAGTCATCTCCCGCTTTAAGA
TTATCTCTAACCTGAATATTGCGGAAAAACGGGTTCCCCAAGATGGCAAGCTCAAGCGTAATTTCCAAGGTCGCCGTGTG
GACTTTCGTGTTAATACCTGTCCTACTCAAAATGGTGAGAAAGTTTGTCTACGGATTCTCGATAACTCCAATACCCAACT
GGGGCTTGATAAATTAATCAGTGATCCCGAAAGTTTGGAACTAATGCAAAGCTTTGCCCAACGCCCCTATGGACTAATAC
TCGTTACAGGTCCTACGGGTTCAGGCAAAACCACCACTCTCTACTCAACCCTCGCAGAATGCAACGATCCTGGCATTAAT
ATTAGTACTGCTGAAGATCCCGTTGAATATAATTTACCTGGCTTAACCCAATGCCAAGTTTTGCGCGAAAAGGGAATGAC
CTTTGCGAGTATTCTCCGCGCCTTTCTGCGTCAAGATCCTGATGTTATTCTTGTGGGCGAGACCCGCGACGCGGAAACAG
CTAAAACAGCGATCGAAGCAGCGCTAACAGGTCACTTAGTTCTGACCACCCTCCATACCAATGATGCGCCTAGTTGTATC
GCCCGTCTTGAAGAAATGGGTGTAGAGCCATTTATGGCAAGTACCGCCATCATTGGTGTACTCGCCCAGCGTTTATTGCG
CCGCATCTGCGACAATTGCCGCATCCCCTATAATCCATCCCAAGAAGAACTTGATCGATTTGGTTTACCAAGTAGCGATC
TCGAAAGAACCTTCTACCGAGCCAACATCGTGAACCGCGATGCTATGCTTCCTGGTCAGCGTGTATGCCCTAAATGTAAC
GGCTCTGGATATAAAGGTCGCGCAGGGGTCTACGAAATCATGAAGATGACTGAACGTCTGCAAAGTGCGATTAACAAGGG
AGCCACTACGGAAGTCATTAAGGAGATTGCCGTACAAGAGGGGATGAAAACCCTGATGGCTTATGCTTTTGATCTTGTCA
GACAAGGGACAACTACCCTTGATGAAGTCCTACGTGTGGTTTATACCGATAAAGGTAGAGAAGCCGAAGAACGTGCTCGG
CGTGGTAAAGGGCTAGAGTGTGATAACTGTGATGCAATGCTCACACCTGAAACCGTCGAATGTCCGTACTGCACTACACC
TAGAACTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-508)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(79-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

51.791

100

0.518