Detailed information NTG0688341

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: M4S28_RS16295
• Genome accession: NZ_CP097293
• Coordinates: 3590011..3591036 (-)
• Length: 341 a.a.
• NCBI ID: WP_102462300.1
• Uniprot ID: -
• Organism: Vibrio sp. J383
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3585011..3596036

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M4S28_RS16270 (M4S28_16270)	aroK	3585852..3586370 (-)	519	WP_004729664.1	shikimate kinase AroK	-
M4S28_RS16275 (M4S28_16275)	pilQ	3586547..3588265 (-)	1719	WP_249632103.1	type IV pilus secretin PilQ family protein	Machinery gene
M4S28_RS16280 (M4S28_16280)	pilP	3588301..3588819 (-)	519	WP_249632742.1	pilus assembly protein PilP	Machinery gene
M4S28_RS16285 (M4S28_16285)	pilO	3588806..3589435 (-)	630	WP_249632104.1	type 4a pilus biogenesis protein PilO	Machinery gene
M4S28_RS16290 (M4S28_16290)	pilN	3589428..3590027 (-)	600	WP_102488973.1	PilN domain-containing protein	Machinery gene
M4S28_RS16295 (M4S28_16295)	pilM	3590011..3591036 (-)	1026	WP_102462300.1	type IV pilus assembly protein PilM	Machinery gene
M4S28_RS16300 (M4S28_16300)	-	3591188..3593749 (+)	2562	WP_249632105.1	PBP1A family penicillin-binding protein	-
M4S28_RS16305 (M4S28_16305)	oxyR	3593862..3594755 (-)	894	WP_017080198.1	DNA-binding transcriptional regulator OxyR	-
M4S28_RS16310 (M4S28_16310)	-	3594754..3594933 (+)	180	WP_017080197.1	hypothetical protein	-
M4S28_RS16315 (M4S28_16315)	-	3594977..3595705 (+)	729	WP_017073712.1	glutathione peroxidase	-

Sequence

Protein

• Length: 341 a.a.
• Molecular weight: 38106.70 Da
• Isoelectric Point: 7.0208

>NTDB_id=688341 M4S28_RS16295 WP_102462300.1 3590011..3591036(-) (pilM) [Vibrio sp. J383]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCCNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
VVVDTQAHGLLNIWQMASRLYPEKKNWLLVDVGIDQTSLGIIPQGLAPFYKDIAFGTKDLRSHGAASDIESVFATTEDIH
QFIVNLIEKLKRQLQLYSSGNAQQPISGIWLMGEGASIPMVTEELERHFQLSCESLNPLSLFENKVAKRRRLPTNWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide

• Length: 1026 bp

>NTDB_id=688341 M4S28_RS16295 WP_102462300.1 3590011..3591036(-) (pilM) [Vibrio sp. J383]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCCGTGGTACTAAAACCCGTGGGGGAGTT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTTAAAGAACTTAGGAAAGGTCTGCCTTTTGGCTGTTGCAACGTCGCCATTTCAGTACCTGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGACAGAGAAAAAGAGTTTTCGATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCGATAGAGGAGCTGAGTTTAGATTTTGTAAAGCTGGAAGACAAACGATTTGGTAAAGGTTCGACAA
GCAGTTATCAGGTATACGCCACTCGTAAAGAAGTGGTGGATAGCCGAGCGGATGCTTTGACCAAAGCTGGTTTTAAACCT
GTGGTGGTAGACACGCAGGCGCATGGGTTGCTTAACATCTGGCAAATGGCATCGCGCCTGTACCCCGAAAAGAAAAATTG
GCTGCTGGTGGATGTGGGAATCGACCAAACGTCGTTAGGCATTATTCCACAAGGTTTGGCACCGTTTTATAAAGATATCG
CTTTTGGTACCAAAGATCTTCGTAGCCATGGTGCTGCAAGTGATATCGAGAGTGTGTTCGCCACCACTGAAGATATACAT
CAATTTATTGTTAATTTGATTGAGAAGCTCAAGCGTCAATTACAGCTCTATTCATCAGGAAATGCACAGCAACCTATCTC
AGGGATCTGGTTGATGGGCGAGGGCGCCAGCATTCCTATGGTAACCGAAGAGCTAGAACGTCATTTTCAGTTGAGTTGCG
AGTCACTGAACCCTTTATCTCTATTTGAGAATAAGGTGGCGAAGCGACGTCGGTTACCAACGAACTGGCAACATTTCGGT
ATTGCGGCGGGTATGGCGATGAGTGGACTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-220)

• Type IV pilus assembly protein PilM; (PilM_2) (240-328)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	53.824	99.707	0.537
pilM	Vibrio campbellii strain DS40M4	47.368	100	0.475