Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   M4S28_RS16275 Genome accession   NZ_CP097293
Coordinates   3586547..3588265 (-) Length   572 a.a.
NCBI ID   WP_249632103.1    Uniprot ID   -
Organism   Vibrio sp. J383     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3581547..3593265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4S28_RS16255 (M4S28_16255) - 3582223..3583071 (-) 849 WP_004729661.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  M4S28_RS16260 (M4S28_16260) - 3583141..3584658 (-) 1518 WP_249632102.1 AAA family ATPase -
  M4S28_RS16265 (M4S28_16265) aroB 3584738..3585826 (-) 1089 WP_004729663.1 3-dehydroquinate synthase -
  M4S28_RS16270 (M4S28_16270) aroK 3585852..3586370 (-) 519 WP_004729664.1 shikimate kinase AroK -
  M4S28_RS16275 (M4S28_16275) pilQ 3586547..3588265 (-) 1719 WP_249632103.1 type IV pilus secretin PilQ family protein Machinery gene
  M4S28_RS16280 (M4S28_16280) pilP 3588301..3588819 (-) 519 WP_249632742.1 pilus assembly protein PilP Machinery gene
  M4S28_RS16285 (M4S28_16285) pilO 3588806..3589435 (-) 630 WP_249632104.1 type 4a pilus biogenesis protein PilO Machinery gene
  M4S28_RS16290 (M4S28_16290) pilN 3589428..3590027 (-) 600 WP_102488973.1 PilN domain-containing protein Machinery gene
  M4S28_RS16295 (M4S28_16295) pilM 3590011..3591036 (-) 1026 WP_102462300.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62005.75 Da        Isoelectric Point: 4.4494

>NTDB_id=688337 M4S28_RS16275 WP_249632103.1 3586547..3588265(-) (pilQ) [Vibrio sp. J383]
MIKGISGLVSKALQGFAVSVMLVFSVLSHAESLPNQLESIDFRVNKDKDAVIIIELATSTAVVDVQRAQEGLSIELLNTQ
VNDDKLYLLDVKDFATLVEGIEVYKETSSTRLLATIAGDYQYEYNLKGRFIEVVISKPVINEATKEKSVLEKEGKLISIN
FQDIPVRNVLQLIADYNDFNLVVSDSVAGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKAELDLREQQALEK
SRLEEELGELKSEIIKINFAKATDIADMIGGEGAVSMLSDRGSITIDERTNSLLIRELEENIAVIRGIIESLDIPVKQVQ
IEARIVTVTEGNLDELGVRWGVSSTNGSFKVGGSIEGNHPSQITPYDDNGGNSAIDDFLNVNLGATSPNASSIAFQVAKL
GSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLD
LSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSSTVDKVPLLGDLPVLGALFRRSYENVGKSE
LLIFVTPKVVIQ

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=688337 M4S28_RS16275 WP_249632103.1 3586547..3588265(-) (pilQ) [Vibrio sp. J383]
ATGATTAAAGGAATAAGTGGATTAGTGAGCAAGGCTCTACAAGGTTTTGCTGTGTCTGTGATGTTGGTCTTTAGTGTGCT
CAGTCACGCCGAAAGCTTACCTAATCAATTGGAGAGTATTGATTTTAGGGTAAACAAAGATAAAGACGCCGTCATTATTA
TTGAGCTGGCAACCAGCACCGCTGTGGTGGATGTTCAACGTGCTCAGGAAGGGTTAAGTATCGAATTACTCAATACTCAA
GTTAATGACGATAAGCTTTATCTTTTGGATGTGAAGGATTTCGCGACCCTAGTGGAAGGTATTGAAGTCTATAAAGAGAC
GTCCAGCACACGACTGTTGGCGACGATCGCTGGTGACTATCAGTATGAATATAACCTAAAAGGTCGTTTCATAGAGGTGG
TGATCAGCAAGCCTGTTATCAATGAAGCGACCAAAGAGAAAAGCGTTTTAGAAAAAGAGGGCAAACTGATATCGATTAAC
TTCCAAGATATCCCGGTACGAAATGTCCTACAGCTGATTGCTGACTACAACGATTTCAATCTCGTCGTGTCTGACTCGGT
AGCTGGTAACCTAACTTTACGTTTAGATGGCGTACCTTGGCAGCAAGTTCTCGACATTATCTTGCAAGTTAAAGGGCTAG
ATAAGCGCGTTGATGGCAATGTTATTTTGGTGGCGCCAAAAGCAGAACTCGATCTTCGAGAGCAACAAGCGTTAGAGAAA
TCTCGCTTGGAAGAGGAGTTAGGGGAGCTTAAGTCGGAAATCATCAAGATTAATTTTGCTAAAGCCACCGACATTGCCGA
CATGATTGGCGGTGAAGGTGCGGTGAGCATGTTGTCCGATCGTGGCTCGATTACCATTGATGAACGCACCAATTCCCTAT
TGATTCGTGAACTGGAAGAAAACATTGCCGTGATACGAGGCATCATTGAATCTTTGGACATCCCCGTGAAGCAAGTCCAG
ATAGAGGCGCGCATTGTAACCGTTACCGAGGGTAACCTTGATGAGTTAGGGGTGCGTTGGGGTGTTTCTTCCACTAACGG
TAGCTTCAAAGTTGGCGGCTCGATAGAGGGCAACCATCCATCGCAAATTACGCCGTATGACGATAATGGTGGGAATAGTG
CGATTGACGATTTCCTCAACGTTAATCTAGGGGCGACATCTCCTAATGCCTCAAGCATTGCGTTTCAGGTGGCCAAACTG
GGTTCAGATACCTTGCTTGATCTTGAATTGTCGGCATTACAACAAGAGTCAAAAGCTGAGATTATTTCCAGCCCACGTTT
AATCACCACCAATAAAAAACCCGCTTATATTGAGCAAGGTACTGAAATTCCTTATTTAGAGTCGTCTTCAAGTGGTGCTA
CTTCGGTCGCATTTAAGAAAGCAGTGTTGAGTCTTAAGGTGACACCACAAATCACGCCCGACAATCGTTTGGTATTGGAT
TTAAGCGTGACCCAAGATAGGCCAGGGCAAGTAGTAAAAACGGGAACGGGTGAGGCTGTTGCCATTGATACTCAACGAAT
AGGGACGCAAGTGCTTGTTAATAATGGTGAAACAGTCGTACTTGGTGGAATATTTCAGCACAGTGTGAGCAGTACAGTGG
ACAAAGTTCCTCTGTTAGGAGACCTGCCAGTTTTGGGTGCATTATTCCGTCGTAGCTATGAAAATGTGGGTAAAAGTGAA
CTACTTATTTTTGTTACACCCAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(253-318)

Bacterial type II and III secretion system protein (Secretin)

(412-570)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

77.7

100

0.78
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.7

100

0.78
  pilQ Vibrio campbellii strain DS40M4

81.25

95.105

0.773
  pilQ Legionella pneumophila strain Lp02

43.04

95.455

0.411
  pilQ Legionella pneumophila strain ERS1305867

43.04

95.455

0.411
  pilQ Pseudomonas aeruginosa PAK

40.966

97.727

0.4
  comQ Acinetobacter baylyi ADP1

39.789

99.301

0.395
  pilQ Acinetobacter baumannii D1279779

37.651

100

0.381
  pilQ Acinetobacter baumannii strain A118

37.651

100

0.381