Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   M4S28_RS16290 Genome accession   NZ_CP097293
Coordinates   3589428..3590027 (-) Length   199 a.a.
NCBI ID   WP_102488973.1    Uniprot ID   -
Organism   Vibrio sp. J383     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3584428..3595027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4S28_RS16265 (M4S28_16265) aroB 3584738..3585826 (-) 1089 WP_004729663.1 3-dehydroquinate synthase -
  M4S28_RS16270 (M4S28_16270) aroK 3585852..3586370 (-) 519 WP_004729664.1 shikimate kinase AroK -
  M4S28_RS16275 (M4S28_16275) pilQ 3586547..3588265 (-) 1719 WP_249632103.1 type IV pilus secretin PilQ family protein Machinery gene
  M4S28_RS16280 (M4S28_16280) pilP 3588301..3588819 (-) 519 WP_249632742.1 pilus assembly protein PilP Machinery gene
  M4S28_RS16285 (M4S28_16285) pilO 3588806..3589435 (-) 630 WP_249632104.1 type 4a pilus biogenesis protein PilO Machinery gene
  M4S28_RS16290 (M4S28_16290) pilN 3589428..3590027 (-) 600 WP_102488973.1 PilN domain-containing protein Machinery gene
  M4S28_RS16295 (M4S28_16295) pilM 3590011..3591036 (-) 1026 WP_102462300.1 type IV pilus assembly protein PilM Machinery gene
  M4S28_RS16300 (M4S28_16300) - 3591188..3593749 (+) 2562 WP_249632105.1 PBP1A family penicillin-binding protein -
  M4S28_RS16305 (M4S28_16305) oxyR 3593862..3594755 (-) 894 WP_017080198.1 DNA-binding transcriptional regulator OxyR -
  M4S28_RS16310 (M4S28_16310) - 3594754..3594933 (+) 180 WP_017080197.1 hypothetical protein -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22984.47 Da        Isoelectric Point: 9.8078

>NTDB_id=688340 M4S28_RS16290 WP_102488973.1 3589428..3590027(-) (pilN) [Vibrio sp. J383]
MLHQINLLPWRDEIRAQHKKRFIHLIILGVIIALAGQWAVGHYFYDQQAKQQARLSYLNRYIAELDRQIQSLKVAEQEHK
AILTRLDVVESLQLGRNKTTDFMNLMPELIPEGVYVDKIKMNGQEIEMSGISDSTARLATMLDNLERSDSLKGVEMHSIV
HNRKRFNKEFQTFKVSFVFSPVSLENTTASRKEKGAHHG

Nucleotide


Download         Length: 600 bp        

>NTDB_id=688340 M4S28_RS16290 WP_102488973.1 3589428..3590027(-) (pilN) [Vibrio sp. J383]
ATGTTGCATCAAATTAACCTGCTGCCGTGGCGTGATGAGATTCGCGCTCAGCACAAGAAGCGCTTTATCCATCTGATCAT
TCTTGGCGTCATCATCGCGCTTGCAGGACAATGGGCGGTTGGTCACTATTTTTACGACCAACAAGCCAAGCAGCAAGCCC
GTCTTAGTTACCTCAATCGATATATTGCCGAGCTCGACCGACAAATTCAGTCATTAAAGGTCGCAGAGCAGGAGCATAAA
GCGATTCTGACCAGGCTTGATGTAGTCGAATCGTTGCAGCTTGGTCGCAACAAGACCACCGACTTTATGAATCTGATGCC
GGAGCTTATTCCTGAAGGTGTGTATGTCGACAAGATAAAGATGAATGGTCAGGAGATTGAAATGTCGGGCATTAGCGACA
GTACTGCTCGTCTCGCTACGATGTTGGACAACCTCGAGCGTTCAGATTCTCTGAAAGGGGTCGAGATGCACTCTATCGTT
CATAACCGCAAGCGCTTCAATAAAGAGTTTCAGACCTTCAAGGTCTCTTTTGTGTTTAGCCCTGTGTCTTTAGAAAACAC
AACCGCGAGCAGAAAAGAGAAGGGGGCGCATCATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

62.5

100

0.628
  pilN Vibrio campbellii strain DS40M4

63.978

93.467

0.598