Detailed information NTG0687893

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M4272_RS25565
• Genome accession: NZ_CP097220
• Coordinates: 5297993..5298793 (-)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain 12
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5292993..5303793

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M4272_RS25545 (M4272_25545)	dksA	5293573..5294028 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
M4272_RS25550 (M4272_25550)	sfsA	5294191..5294907 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
M4272_RS25555 (M4272_25555)	thpR	5294907..5295443 (-)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
M4272_RS25560 (M4272_25560)	hrpB	5295516..5297945 (+)	2430	WP_004177388.1	ATP-dependent helicase HrpB	-
M4272_RS25565 (M4272_25565)	pilD	5297993..5298793 (-)	801	WP_004145898.1	A24 family peptidase	Machinery gene
M4272_RS25570 (M4272_25570)	-	5298790..5299536 (-)	747	WP_004178605.1	type II secretion system protein N	-
M4272_RS25575 (M4272_25575)	-	5299526..5300011 (-)	486	WP_004145896.1	type II secretion system protein M	-
M4272_RS25580 (M4272_25580)	gspL	5300004..5301197 (-)	1194	WP_004194919.1	type II secretion system protein GspL	-
M4272_RS25585 (M4272_25585)	gspK	5301184..5302170 (-)	987	WP_004178610.1	type II secretion system minor pseudopilin GspK	-
M4272_RS25590 (M4272_25590)	gspJ	5302167..5302763 (-)	597	WP_004178612.1	type II secretion system minor pseudopilin GspJ	-
M4272_RS25595 (M4272_25595)	gspI	5302760..5303125 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
M4272_RS25600 (M4272_25600)	gspH	5303122..5303637 (-)	516	WP_004194939.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=687893 M4272_RS25565 WP_004145898.1 5297993..5298793(-) (pilD) [Klebsiella pneumoniae strain 12]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=687893 M4272_RS25565 WP_004145898.1 5297993..5298793(-) (pilD) [Klebsiella pneumoniae strain 12]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCATGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425